التفاصيل البيبلوغرافية
| العنوان: |
A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo |
| المؤلفون: |
Sakamoto, Masayuki, Inoue, Masatoshi, Takeuchi, Atsuya, Kobari, Shigetaka, Yokoyama, Tatsushi, Horigane, Shin-ichiro, Takemoto-Kimura, Sayaka, Abe, Manabu, Sakimura, Kenji, Kano, Masanobu, Kitamura, Kazuo, Fujii, Hajime, Bito, Haruhiko |
| المساهمون: |
坂本, 雅行, 井上, 昌俊, 竹内, 敦也, 小針, 成敬, 横山, 達士, 堀金, 慎一郎, 竹本(木村), さやか, 阿部, 学, 崎村, 建司, 狩野, 方伸, 喜多村, 和郎, 藤井, 哉, 尾藤, 晴彦, 00777865 |
| بيانات النشر: |
Elsevier BV |
| سنة النشر: |
2022 |
| المجموعة: |
Kyoto University Research Information Repository (KURENAI) / 京都大学学術情報リポジトリ |
| مصطلحات موضوعية: |
genetically encoded calcium indicator, transgenic mouse, Flp/Frt recombination, in vivo calcium imaging, two-photon microscopy, dual-color imaging |
| الوصف: |
脳の神経活動を可視化する新規マウス系統を開発 --高感度・高速カルシウムセンサーによる神経活動の計測に成功--. 京都大学プレスリリース. 2022-02-15. ; Genetically encoded calcium indicators (GECIs) are widely used to measure calcium transients in neuronal somata and processes, and their use enables the determination of action potential temporal series in a large population of neurons. Here, we generate a transgenic mouse line expressing a highly sensitive green GECI, G-CaMP9a, in a Flp-dependent manner in excitatory and inhibitory neuronal subpopulations downstream of a strong CAG promoter. Combining this reporter mouse with viral or mouse genetic Flp delivery methods produces a robust and stable G-CaMP9a expression in defined neuronal populations without detectable detrimental effects. In vivo two-photon imaging reveals spontaneous and sensory-evoked calcium transients in excitatory and inhibitory ensembles with cellular resolution. Our results show that this reporter line allows long-term, cell-type-specific investigation of neuronal activity with enhanced resolution in defined populations and facilitates dissecting complex dynamics of neural networks in vivo. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| تدمد: |
2667-2375 |
| Relation: |
https://www.kyoto-u.ac.jp/ja/research-news/2022-02-15; http://hdl.handle.net/2433/268236; Cell Reports Methods; 100168 |
| الاتاحة: |
http://hdl.handle.net/2433/268236 |
| Rights: |
© 2022 The Author(s). ; This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.D36D062 |
| قاعدة البيانات: |
BASE |
Full Text Finder