Academic Journal
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi
| العنوان: | Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi |
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| المؤلفون: | Fisher, Matthew C., Ghosh, Pria, Shelton, Jennifer M. G., Bates, Kieran, Brookes, Lola, Wierzbicki, Claudia, Rosa, Gonçalo M., Farrer, Rhys A., Aanensen, David M., Alvarado-Rybak, Mario, Bataille, Arnaud, Berger, Lee, Böll, Susanne, Bosch, Jaime, Clare, Frances C., A. Courtois, Elodie, Crottini, Angelica, Cunningham, Andrew A., Doherty-Bone, Thomas M., Gebresenbet, Fikirte, Gower, David J., Höglund, Jacob, James, Timothy Y., Jenkinson, Thomas S., Kosch, Tiffany A., Lambertini, Carolina, Laurila, Anssi, Lin, Chun-Fu, Loyau, Adeline, Martel, An, Meurling, Sara, Miaud, Claude, Minting, Pete, Ndriantsoa, Serge, O’Hanlon, Simon J., Pasmans, Frank, Rakotonanahary, Tsanta, Rabemananjara, Falitiana C. E., Ribeiro, Luisa P., Schmeller, Dirk S., Schmidt, Benedikt R., Skerratt, Lee, Smith, Freya, Soto-Azat, Claudio, Tessa, Giulia, Toledo, Luís Felipe, Valenzuela-Sánchez, Andrés, Verster, Ruhan, Vörös, Judit, Waldman, Bruce, Webb, Rebecca J., Weldon, Che, Wombwell, Emma, Zamudio, Kelly R., Longcore, Joyce E., Garner, Trenton W. J. |
| بيانات النشر: | Nature |
| سنة النشر: | 2024 |
| المجموعة: | Universidade de Lisboa: repositório.UL |
| الوصف: | Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens. ; info:eu-repo/semantics/publishedVersion |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Biodiversa project RACE: Risk Assessment of Chytridiomycosis to European Amphibian Biodiversity (NERC standard grant NE/K014455/1 and NE/E006701/1; ANR-08-BDVA-002-03); Leverhulme Trust (RPG-2014-273); Morris Animal Foundation; Bolyai János Research Grant of the Hunagrian Academy of Sciences (BO/00597/14); Conservation Leadership Programme Future Conservationist Award; Fondecyt (No. 1181758); Mohamed bin Zayed Species Conservation Fund Project (152510704); FCT SFRH/ BD/69194/2010; São Paulo Research Foundation (FAPESP #2016/25358-3); National Counsel of Technological and Scientifc Development (CNPq #300896/2016–6); Catalyzing New International Collaborations grant from the United States NSF (OISE-1159513); Royal Geographical Society and the Royal Zoological Society of Scotland; National Research Foundation of Korea (2015R1D1A1A01057282); Fisher, M. C., Ghosh, P., Shelton, J., Bates, K. A., Brookes, L., Wierzbicki, C., Rosa, G. M., Farrer, R. A., Aanensen, D. M., Alvarado‐Rybak, M., Bataillé, A., Berger, L., Böll, S., Bosch, J., Clare, F. C., Courtois, E. A., Crottini, A., Cunningham, A. A., Doherty‐Bone, T. M., . . . Garner, T. W. J. (2018). Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-24472-2; http://hdl.handle.net/10451/62355 |
| DOI: | 10.1038/s41598-018-24472-2 |
| الاتاحة: | http://hdl.handle.net/10451/62355 https://doi.org/10.1038/s41598-018-24472-2 |
| Rights: | openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.D3330758 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-018-24472-2 |
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