Raw Confocal Images for 'A quantitative gibberellin signaling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem'

التفاصيل البيبلوغرافية
العنوان: Raw Confocal Images for 'A quantitative gibberellin signaling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem'
المؤلفون: Shi, Bihai, Felipo-Benavent, Amelia, Cerutti, Guillaume, Galvan-Ampudia, Carlos, Jilli, Lucas, Brunoud, Geraldine, Mutterer, Jérome, Vallet, Elody, Sakvarelidze-Achard, Lali, Davière, Jean-Michel, Navarro-Galiano, Alejandro, Walia, Ankit, Lazary, Shani, Legrand, Jonathan, Weinstain, Roy, Jones, Alexander, Prat, Salomé, Achard, Patrick, Vernoux, Teva
المصدر: Nature Communications, 15, 3895 (2024), (2024-05-09)
بيانات النشر: Zenodo
سنة النشر: 2024
المجموعة: Zenodo
الوصف: Growth at the shoot apical meristem (SAM) is essential for shoot architectureconstruction. The phytohormones gibberellins (GA) play a pivotal role incoordinating plant growth, but their role in the SAM remainsmostly unknown.Here, we developed a ratiometric GA signaling biosensor by engineering oneof the DELLA proteins, to suppress its master regulatory function in GA transcriptionalresponses while preserving its degradation upon GA sensing. Wedemonstrate that this degradation-based biosensor accurately reports oncellular changes inGA levels and perception during development.Weused thisbiosensor to map GA signaling activity in the SAM. We show that high GAsignaling is found primarily in cells located between organ primordia that arethe precursors of internodes. By gain- and loss-of-function approaches, wefurther demonstrate that GAs regulate cell division plane orientation toestablish the typical cellular organization of internodes, thus contributing tointernode speci fi cation in the SAM.
نوع الوثيقة: other/unknown material
اللغة: unknown
Relation: https://doi.org/10.1038/s41467-024-48116-4; https://doi.org/10.5281/zenodo.10934410; https://doi.org/10.5281/zenodo.10934411; oai:zenodo.org:10934411
DOI: 10.5281/zenodo.10934411
الاتاحة: https://doi.org/10.5281/zenodo.10934411
Rights: info:eu-repo/semantics/openAccess ; Creative Commons Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/legalcode
رقم الانضمام: edsbas.D3049A10
قاعدة البيانات: BASE