Academic Journal
Measuring Plant Protein Secretion
| العنوان: | Measuring Plant Protein Secretion |
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| المؤلفون: | Larson, Emily R |
| المصدر: | Larson , E R 2017 , ' Measuring Plant Protein Secretion ' , Methods in Molecular Biology , vol. 1662 , pp. 199-207 . https://doi.org/10.1007/978-1-4939-7262-3_18 |
| سنة النشر: | 2017 |
| المجموعة: | University of Bristol: Bristol Reserach |
| مصطلحات موضوعية: | Agrobacterium tumefaciens/genetics, Arabidopsis/drug effects, Arabidopsis Proteins/genetics, Dexamethasone/pharmacology, Gene Expression, Genes, Reporter, Green Fluorescent Proteins/genetics, Luminescent Proteins/genetics, Oligopeptides/genetics, Optical Imaging/methods, Plant Leaves/drug effects, Plant Roots/drug effects, Plants, Genetically Modified, Plasmids/chemistry, Promoter Regions, Genetic, Protein Sorting Signals/genetics, Protein Transport, Qa-SNARE Proteins/genetics, Recombinant Fusion Proteins/genetics, Seedlings/drug effects, Transformation, Transport Vesicles/metabolism, Vacuoles/metabolism |
| الوصف: | Fluorescent protein fusions are widely used for visualizing the subcellular localization and mobility of intercellular proteins. There is now a variety of colors, expression vectors, and photoactivated molecules to choose from, each with their own strengths and limitations. In this chapter, the methodologies for expressing and quantifying protein secretion with fluorescent protein fusion constructs using two separate protocols-one in which the retention of a transiently expressed fluorescent marker is measured in seedling roots to quantify a block in secretion, and one in which the secretion of a fluorescent marker into the space of the apoplast is measured to quantify secretion in plant leaves-are described. In the first protocol, seedling roots are transiently transformed with multicistronic constructs; and in the second protocol, markers can be stably expressed and controlled under an inducible promoter in mature plants. Both methods provide tools for quantifying protein secretion and visualizing defects in secretion pathways in Arabidopsis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1007/978-1-4939-7262-3_18 |
| الاتاحة: | https://hdl.handle.net/1983/0531be7e-adfd-404e-a93e-0da928f923e7 https://research-information.bris.ac.uk/en/publications/0531be7e-adfd-404e-a93e-0da928f923e7 https://doi.org/10.1007/978-1-4939-7262-3_18 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.D3000E1E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/978-1-4939-7262-3_18 |
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