Academic Journal
Discovery of putative long non-coding RNAs expressed in the eyes of Astyanax mexicanus (Actinopterygii: Characidae)
| العنوان: | Discovery of putative long non-coding RNAs expressed in the eyes of Astyanax mexicanus (Actinopterygii: Characidae) |
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| المؤلفون: | Batista da Silva, Iuri, Aciole Barbosa, David, Kavalco, Karine Frehner, Nunes, Luiz R., Pasa, Rubens, Menegidio, Fabiano B. |
| المصدر: | Scientific Reports ; volume 13, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| الوصف: | Astyanax mexicanus is a well-known model species, that has two morphotypes, cavefish, from subterranean rivers and surface fish, from surface rivers. They are morphologically distinct due to many troglomorphic traits in the cavefish, such as the absence of eyes. Most studies on A . mexicanus are focused on eye development and protein-coding genes involved in the process. However, lncRNAs did not get the same attention and very little is known about them. This study aimed to fill this knowledge gap, identifying, describing, classifying, and annotating lncRNAs expressed in the embryo’s eye tissue of cavefish and surface fish. To do so, we constructed a concise workflow to assemble and evaluate transcriptomes, annotate protein-coding genes, ncRNAs families, predict the coding potential, identify putative lncRNAs, map them and predict interactions. This approach resulted in the identification of 33,069 and 19,493 putative lncRNAs respectively mapped in cavefish and surface fish. Thousands of these lncRNAs were annotated and identified as conserved in human and several species of fish. Hundreds of them were validated in silico, through ESTs. We identified lncRNAs associated with genes related to eye development. This is the case of a few lncRNAs associated with sox2 , which we suggest being isomorphs of the SOX2-OT , a lncRNA that can regulate the expression of sox2 . This work is one of the first studies to focus on the description of lncRNAs in A . mexicanus , highlighting several lncRNA targets and opening an important precedent for future studies focusing on lncRNAs expressed in A . mexicanus . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-023-34198-5 |
| الاتاحة: | http://dx.doi.org/10.1038/s41598-023-34198-5 https://www.nature.com/articles/s41598-023-34198-5.pdf https://www.nature.com/articles/s41598-023-34198-5 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.D2FB437C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-023-34198-5 |
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