التفاصيل البيبلوغرافية
| العنوان: |
Table_3_A Multi-Omics Analysis of Mucosal-Associated-Invariant T Cells Reveals Key Drivers of Distinct Modes of Activation.xlsx |
| المؤلفون: |
Kristin Schubert (7462043), Isabel Karkossa (7549370), Jana Schor (8041853), Beatrice Engelmann (6029429), Lisa Maria Steinheuer (10866081), Tony Bruns (3632902), Ulrike Rolle-Kampczyk (832720), Jörg Hackermüller (39435), Martin von Bergen (6860861) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, MAIT cells, multi-omics analysis, TCR-dependent, TCR-independent, activation, key driver analysis |
| الوصف: |
The function of mucosal-associated invariant T (MAIT) cells highly depends on the mode of activation, either by recognition of bacterial metabolites via their T cell receptor (TCR) or in a TCR-independent manner via cytokines. The underlying molecular mechanisms are not entirely understood. To define the activation of MAIT cells on the molecular level, we applied a multi-omics approach with untargeted transcriptomics, proteomics and metabolomics. Transcriptomic analysis of E. coli- and TCR-activated MAIT cells showed a distinct transcriptional reprogramming, including altered pathways, transcription factors and effector molecules. We validated the consequences of this reprogramming on the phenotype by proteomics and metabolomics. Thus, and to distinguish between TCR-dependent and -independent activation, MAIT cells were stimulated with IL12/IL18, anti-CD3/CD28 or both. Only a combination of both led to full activation of MAIT cells, comparable to activation by E. coli. Using an integrated network-based approach, we identified key drivers of the distinct modes of activation, including cytokines and transcription factors, as well as negative feedback regulators like TWIST1 or LAG3. Taken together, we present novel insights into the biological function of MAIT cells, which may represent a basis for therapeutic approaches to target MAIT cells in pathological conditions. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Table_3_A_Multi-Omics_Analysis_of_Mucosal-Associated-Invariant_T_Cells_Reveals_Key_Drivers_of_Distinct_Modes_of_Activation_xlsx/14666112 |
| DOI: |
10.3389/fimmu.2021.616967.s010 |
| الاتاحة: |
https://doi.org/10.3389/fimmu.2021.616967.s010 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.D2FAD56E |
| قاعدة البيانات: |
BASE |