Academic Journal
Structural requirements for calmodulin binding to membrane‐associated guanylate kinase homologs
| العنوان: | Structural requirements for calmodulin binding to membrane‐associated guanylate kinase homologs |
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| المؤلفون: | Paarmann, Ingo, Lye, Ming F., Lavie, Arnon, Konrad, Manfred |
| المصدر: | Protein Science ; volume 17, issue 11, page 1946-1954 ; ISSN 0961-8368 1469-896X |
| بيانات النشر: | Wiley |
| سنة النشر: | 2008 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Effector molecules such as calmodulin modulate the interactions of membrane‐associated guanylate kinase homologs (MAGUKs) and other scaffolding proteins of the membrane cytoskeleton by binding to the Src homology 3 (SH3) domain, the guanylate kinase (GK) domain, or the connecting HOOK region of MAGUKs. Using surface plasmon resonance, we studied the interaction of members of all four MAGUK subfamilies—synapse‐associated protein 97 (SAP97), calcium/calmodulin‐dependent serine protein kinase (CASK), membrane palmitoylated protein 2 (MPP2), and zona occludens (ZO) 1—and calmodulin to determine interaction affinities and localize the binding site. The SH3‐GK domains of the proteins and derivatives thereof were expressed in E. coli and purified. In all four proteins, high‐affinity calmodulin binding was identified. CASK was shown to contain a Ca 2+ ‐dependent calmodulin binding site within the HOOK region, overlapping with a protein 4.1 binding site. In ZO1, a Ca 2+ ‐dependent calmodulin binding site was detected within the GK domain. The equilibrium dissociation constants for MAGUK–calmodulin interaction were found to range from 50 nM to 180 nM. Sequence analyses suggest that binding sites for calmodulin have evolved independently in at least three subfamilies. For ZO1, pulldown of GST‐calmodulin was shown to occur in a calcium‐dependent manner; moreover, molecular modeling and sequence analyses predict conserved basic residues to be exposed on one side of a helix. Thus, calmodulin binding appears to be a common feature of MAGUKs, and Ca 2+ ‐activated calmodulin may serve as a general regulator to affect the interactions of MAGUKs and various components of the cytoskeleton. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1110/ps.035550.108 |
| الاتاحة: | http://dx.doi.org/10.1110/ps.035550.108 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1110%2Fps.035550.108 https://onlinelibrary.wiley.com/doi/pdf/10.1110/ps.035550.108 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.D2CD8088 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1110/ps.035550.108 |
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