Academic Journal
The Structure of a Plant Tyrosinase from Walnut Leaves Reveals the Importance of 'Substrate-Guiding Residues' for Enzymatic Specificity
| العنوان: | The Structure of a Plant Tyrosinase from Walnut Leaves Reveals the Importance of 'Substrate-Guiding Residues' for Enzymatic Specificity |
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| المؤلفون: | Bijelic, Aleksandar, Pretzler, Matthias, Molitor, Christian, Zekiri, Florime, Rompel, Annette |
| المصدر: | Angewandte Chemie / International edition 54, 14677 - 14680 (2015). doi:10.1002/anie.201506994 |
| بيانات النشر: | Wiley-VCH |
| سنة النشر: | 2015 |
| المجموعة: | DESY Publication Database (PUBDB) |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/540 |
| جغرافية الموضوع: | DE |
| الوصف: | Tyrosinases and catechol oxidases are members of the class of type III copper enzymes. While tyrosinases accept both mono- and o-diphenols as substrates, only the latter substrate is converted by catechol oxidases. Researchers have been working for decades to elucidate the monophenolase/diphenolase specificity on a structural level and have introduced an early hypothesis that states that the reason for the lack of monophenolase activity in catechol oxidases may be its structurally restricted active site. However, recent structural and biochemical studies of this enzyme class have raised doubts about this theory. Herein, the first crystal structure of a plant tyrosinase (from Juglans regia) is presented. The structure reveals that the distinction between mono- and diphenolase activity does not depend on the degree of restriction of the active site, and thus a more important role for amino acid residues located at the entrance to and in the second shell of the active site is proposed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/issn/0044-8249; info:eu-repo/semantics/altIdentifier/issn/0570-0833; info:eu-repo/semantics/altIdentifier/issn/1521-3773; info:eu-repo/semantics/altIdentifier/wos/WOS:000367723400008; info:eu-repo/semantics/altIdentifier/issn/1433-7851; info:eu-repo/semantics/altIdentifier/pmid/pmid:26473311; https://bib-pubdb1.desy.de/record/296513; https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2016-01515%22 |
| الاتاحة: | https://bib-pubdb1.desy.de/record/296513 https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2016-01515%22 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D2920D49 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |