Academic Journal
A monoclonal antibody for G protein-coupled receptor crystallography
| العنوان: | A monoclonal antibody for G protein-coupled receptor crystallography |
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| المؤلفون: | Day, Peter W, Rasmussen, Søren Gøgsig Faarup, Parnot, Charles, Fung, Juan José, Masood, Asna, Kobilka, Tong Sun, Yao, Xiao-Jie, Choi, Hee-Jung, Weis, William I, Rohrer, Daniel K, Kobilka, Brian K |
| المصدر: | Day , P W , Rasmussen , S G F , Parnot , C , Fung , J J , Masood , A , Kobilka , T S , Yao , X-J , Choi , H-J , Weis , W I , Rohrer , D K & Kobilka , B K 2007 , ' A monoclonal antibody for G protein-coupled receptor crystallography ' , Nature Methods , vol. 4 , no. 11 , pp. 927-9 . https://doi.org/10.1038/nmeth1112 |
| سنة النشر: | 2007 |
| المجموعة: | University of Copenhagen: Research / Forskning ved Københavns Universitet |
| مصطلحات موضوعية: | Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigen-Antibody Reactions, Blotting, Western, Crystallization, Crystallography, Epitopes, Fluorescent Dyes, Humans, Immunoglobulin Fab Fragments, Mice, Molecular Sequence Data, Protein Structure, Tertiary, Receptors, Adrenergic, beta-2, G-Protein-Coupled, Recombinant Proteins, Rhodamines, Vaccination |
| الوصف: | G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/nmeth1112 |
| الاتاحة: | https://researchprofiles.ku.dk/da/publications/a-monoclonal-antibody-for-g-proteincoupled-receptor-crystallography(3046f264-75f4-463a-b0b8-1a2d394cefcc).html https://doi.org/10.1038/nmeth1112 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.D27CFDE3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/nmeth1112 |
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