التفاصيل البيبلوغرافية
| العنوان: |
Comparison of a Putative Second Serotype of Chicken Infectious Anemia Virus with a Prototypical Isolate II. Antigenic and Physicochemical Characteristics |
| المؤلفون: |
E. Spackman, S. S. Cloud, J. K. Rosenberger |
| المساهمون: |
E. Spackman, S. S. Cloud, J. K. Rosenberger |
| المصدر: |
https://doi.org/10.1637/0005-2086(2002)046[0956:COAPSS]2.0.CO;2. |
| بيانات النشر: |
American Association of Avian Pathologists |
| سنة النشر: |
2002 |
| المجموعة: |
BioOne Online Journals |
| جغرافية الموضوع: |
world |
| الوصف: |
A putative new serotype of chicken infectious anemia virus (CIAV) isolated from 17-wk-old broiler breeder pullets was compared with a known, previously characterized CIAV isolate, the Del-Ros strain. Physicochemical characteristics evaluated included thermal stability, size, pH, and chloroform sensitivity. Physicochemically, CIAV-7 was identical to CIAV. The virus isolates were compared antigenically by enzyme-linked immunosorbent assay, virus neutralization, immunofluorescence assay, and western blot. All four serologic assays demonstrated that CIAV-7 is antigenically distinct from the Del-Ros strain of CIAV. Additionally, polymerase chain reaction (PCR) and Southern blot were used to determine if there were similarities in genome sequence between the two viruses. CIAV-7 could not be detected with CIAV-specific PCR primers or a with CIAV-specific probe by Southern hybridization. |
| نوع الوثيقة: |
text |
| وصف الملف: |
text/HTML |
| اللغة: |
English |
| DOI: |
10.1637/0005-2086(2002)046[0956:COAPSS]2.0.CO;2 |
| الاتاحة: |
https://doi.org/10.1637/0005-2086(2002)046[0956:COAPSS]2.0.CO;2 |
| Rights: |
All rights reserved. |
| رقم الانضمام: |
edsbas.D2640B45 |
| قاعدة البيانات: |
BASE |