Academic Journal
p HBMT1, a BAHD-family monolignol acyltransferase, mediates lignin acylation in poplar
| العنوان: | p HBMT1, a BAHD-family monolignol acyltransferase, mediates lignin acylation in poplar |
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| المؤلفون: | de Vries, Lisanne, MacKay, Heather A, Smith, Rebecca A, Mottiar, Yaseen, Karlen, Steven D, Unda, Faride, Muirragui, Emilia, Bingman, Craig, Vander Meulen, Kirk, Beebe, Emily T, Fox, Brian G, Ralph, John, Mansfield, Shawn D |
| المساهمون: | US Department of Energy, Office of Science, Great Lakes Bioenergy Research Center |
| المصدر: | Plant Physiology ; volume 188, issue 2, page 1014-1027 ; ISSN 0032-0889 1532-2548 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2021 |
| الوصف: | Poplar (Populus) lignin is naturally acylated with p-hydroxybenzoate ester moieties. However, the enzyme(s) involved in the biosynthesis of the monolignol–p-hydroxybenzoates have remained largely unknown. Here, we performed an in vitro screen of the Populus trichocarpa BAHD acyltransferase superfamily (116 genes) using a wheatgerm cell-free translation system and found five enzymes capable of producing monolignol–p-hydroxybenzoates. We then compared the transcript abundance of the five corresponding genes with p-hydroxybenzoate concentrations using naturally occurring unrelated genotypes of P. trichocarpa and revealed a positive correlation between the expression of p-hydroxybenzoyl-CoA monolig-nol transferase (pHBMT1, Potri.001G448000) and p-hydroxybenzoate levels. To test whether pHBMT1 is responsible for the biosynthesis of monolignol–p-hydroxybenzoates, we overexpressed pHBMT1 in hybrid poplar (Populus alba × P. grandidentata) (35S::pHBMT1 and C4H::pHBMT1). Using three complementary analytical methods, we showed that there was an increase in soluble monolignol–p-hydroxybenzoates and cell-wall-bound monolignol–p-hydroxybenzoates in the poplar transgenics. As these pendent groups are ester-linked, saponification releases p-hydroxybenzoate, a precursor to parabens that are used in pharmaceuticals and cosmetics. This identified gene could therefore be used to engineer lignocellulosic biomass with increased value for emerging biorefinery strategies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/plphys/kiab546 |
| DOI: | 10.1093/plphys/kiab546/42010485/kiab546.pdf |
| الاتاحة: | http://dx.doi.org/10.1093/plphys/kiab546 https://academic.oup.com/plphys/advance-article-pdf/doi/10.1093/plphys/kiab546/42010485/kiab546.pdf https://academic.oup.com/plphys/article-pdf/188/2/1014/45890100/kiab546.pdf |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.D2591F0C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/plphys/kiab546 |
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