Academic Journal
Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori
| العنوان: | Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori |
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| المؤلفون: | Celik, Cagla, Sezgin, Gulten Can, Kocabas, Umut Gorkem, Gursoy, Sebnem, Ildiz, Nilay, Tan, Weihong, Ocsoy, Ismail |
| المساهمون: | Belirlenecek |
| بيانات النشر: | Amer Chemical Soc |
| سنة النشر: | 2021 |
| المجموعة: | Hitit University Institutional Repository (DSpace@Hitit) |
| مصطلحات موضوعية: | [No Keywords] |
| الوصف: | Several different diagnostic tests have been reported for rapid, sensitive, and economical detection of bacterial pathogens, but most lack widespread and practical use in the clinic. In this study, we used anthocyanins from red cabbage (Brassica oleracea) as a natural pH indicator and, for the first time, incorporated this agent into a simple, rapid, and economical colorimetric strategy for the detection of Helicobacter pylori (H. pylori) (RCE@test). We prepared two sets of RCE@test solutions (test 1 is purple, and test 2 is blue) in different forms, including liquid, adsorbed filter paper, and agar, and investigated the performance of each RCE@test as a function of the test volume, H. pylori concentration, and reaction time. To elucidate the effect of the pathophysiological environment on these RCE@tests, H. pylori in an artificial gastric fluid was also detected. The 10 and 1 CFU/mL H. pylori suspensions were detected in 15 min and 3 h, respectively, and the limit of detection was determined down to 1 CFU/mL. We experimentally demonstrated the advantages of the RCE@test for detection of H. pylori by comparing it to a commercially available rapid urease test, the CLO test (Campylobacter-like organism test). In addition to colorimetric detection by the naked eyes, RGB (Red Green Blue) and Delta-E analysis in image-processing software was run to quantitatively monitor changes of color in the RCE@test using a smartphone application. Finally, we propose that this test provides simple, effective, rapid, and inexpensive detection and that it can be easily implemented for clinical use. ; 2020 BAGEP Award of the Science Academy; Turkish Academy of Sciences (2018 TUBA GEBIP Award) ; This work was supported by the 2020 BAGEP Award of the Science Academy and Turkish Academy of Sciences (2018 TUBA GEBIP Award). We would like to thank Mucahit Osman Turkan and Busra Aydin for their assistance in drawing and designing TOC. ; WOS:000643539400033 ; 2-s2.0-85104943598 ; PubMed: 33825433 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0003-2700 1520-6882 |
| Relation: | Analytical Chemistry; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.1021/acs.analchem.1c00663; https://hdl.handle.net/11491/7497; 93; 15; 6246; 6253 |
| DOI: | 10.1021/acs.analchem.1c00663 |
| الاتاحة: | https://hdl.handle.net/11491/7497 https://doi.org/10.1021/acs.analchem.1c00663 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.D1F3192E |
| قاعدة البيانات: | BASE |
| تدمد: | 00032700 15206882 |
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| DOI: | 10.1021/acs.analchem.1c00663 |