التفاصيل البيبلوغرافية
| العنوان: |
Cryo Soft X ray Tomography of Whole Cells in the Laboratory |
| المؤلفون: |
Fahy, Kenneth, Kapishnikov, Sergey, Fyans, William, Skoko, Dunja, O'Reilly, Fergal, McEnroe, Tony, Sheridan, Paul |
| بيانات النشر: |
Zenodo |
| سنة النشر: |
2022 |
| المجموعة: |
Zenodo |
| مصطلحات موضوعية: |
soft X-ray, cryo-tomography, tomography, organelles, laboratory soft X-ray microscope |
| الوصف: |
SiriusXT has developed a commercial bench top cryo soft X ray microscope (the SXT-100) for 3 D cryo soft X ray tomography. Cryo SXT uses X rays in the ‘water window’ that extends from the K absorption edge of carbon to the K edge of oxygen, that is from about 282 eV (λ 4 4 nm) to 533 eV (λ 2 3 nm) Water is transparent to these X rays, but organic molecules are absorbing Therefore, these X rays can be used as the basis for microscopy of whole cells in their near native ( state, without need for any contrast enhancing agents A 3 D tomogram with resolution 50 nm (full pitch) can be produced by rotating the cell over a range of angles, with an image acquired at each tilt angle.The concept is equivalent to a medical CT scan applied at the nanoscale |
| نوع الوثيقة: |
conference object |
| اللغة: |
unknown |
| Relation: |
https://zenodo.org/communities/eu; https://doi.org/10.5281/zenodo.7188367; https://doi.org/10.5281/zenodo.7188368; oai:zenodo.org:7188368 |
| DOI: |
10.5281/zenodo.7188368 |
| الاتاحة: |
https://doi.org/10.5281/zenodo.7188368 |
| Rights: |
info:eu-repo/semantics/openAccess ; Creative Commons Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/legalcode |
| رقم الانضمام: |
edsbas.D14C1FFD |
| قاعدة البيانات: |
BASE |