Academic Journal
Uninfected bloodmeal induces hemolymph APL1C protein.
| العنوان: | Uninfected bloodmeal induces hemolymph APL1C protein. |
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| المؤلفون: | Natalia Marta Zmarlak, Catherine Lavazec, Emma Brito-Fravallo, Corinne Genève, Eduardo Aliprandini, Manuela Camille Aguirre-Botero, Kenneth D. Vernick, Christian Mitri |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Microbiology, Cell Biology, Molecular Biology, Immunology, Cancer, Infectious Diseases, Computational Biology, Physical Sciences not elsewhere classified, extracellular space exposed, based receptors used, immune effector activity, first functional explanation, div >< p, human malaria parasite, mosquito remains unclear, plasmodium falciparum <, plasmodium berghei <, malaria ookinete killing, like tep1 protein, parasite immune recognition, lrim1 bind specifically, plasmodium <, p <, berghei <, immune protection, tep1 transcript, ookinete killing, functional counterparts, pattern recognition, anopheles < |
| الوصف: | A . Immunoblot analysis of APL1C protein level in hemolymph after NBM. Mosquito hemolymph was collected at 24 h post-NBM and analyzed by western blot using APL1C antibody in three biological replicates, with hemolymph from UF mosquitoes as the control. The APL1C protein bands quantified as a unit are indicated by the red bracket, protein size ladder (kD) shown in green on the left of protein samples. B . Hemolymph APL1C levels quantified by densitometry of western blots. First, for each sample, the specific APL1C signal was determined by subtracting the background signal of an adjacent same-size empty area (adjusted volume intensity, Adj. Vol. Int.). Secondly, the NBM/UF ratio of APL1C protein levels (Rel. Quant,) was calculated for each biological replicate. C . The XZ and YZ orthogonal confocal views of the NBM stack picture show that APL1C protein (red) localizes extracellularly, on the basal side (ba) and not lumen side (lu) of the midgut surface. D . Midgut basal lamina is permeable to a control molecule of similar molecular mass to APL1C protein. Confocal imaging analysis of midguts from mosquitoes injected with 70 kDa fluorescent dextran polymer indicates that dextran (red) diffuses through basal lamina and is captured on fixed midguts, with an appearance to immunostained APL1C on fixed midguts. For C and D nuclei and actin were stained with DAPI and Phalloidin (blue), respectively and the scale bar is 10 μm. (PDF) |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Uninfected_bloodmeal_induces_hemolymph_APL1C_protein_/25221842 |
| DOI: | 10.1371/journal.ppat.1012008.s001 |
| الاتاحة: | https://doi.org/10.1371/journal.ppat.1012008.s001 https://figshare.com/articles/journal_contribution/Uninfected_bloodmeal_induces_hemolymph_APL1C_protein_/25221842 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.D138EEDE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.ppat.1012008.s001 |
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