Academic Journal
In vitro exposure to isoprene-derived secondary organic aerosol by direct deposition and its effects on COX-2 and IL-8 gene expression
| العنوان: | In vitro exposure to isoprene-derived secondary organic aerosol by direct deposition and its effects on COX-2 and IL-8 gene expression |
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| المؤلفون: | Arashiro, Maiko, Lin, Ying-Hsuan, Sexton, Kenneth G., Zhang, Zhenfa, Jaspers, Ilona, Fry, Rebecca C., Vizuete, William G., Gold, Avram, Surratt, Jason D. |
| المصدر: | eISSN: 1680-7324 |
| سنة النشر: | 2018 |
| المجموعة: | Copernicus Publications: E-Journals |
| الوصف: | Atmospheric oxidation of isoprene, the most abundant non-methane hydrocarbon emitted into Earth's atmosphere primarily from terrestrial vegetation, is now recognized as a major contributor to the global secondary organic aerosol (SOA) burden. Anthropogenic pollutants significantly enhance isoprene SOA formation through acid-catalyzed heterogeneous chemistry of epoxide products. Since isoprene SOA formation as a source of fine aerosol is a relatively recent discovery, research is lacking on evaluating its potential adverse effects on human health. The objective of this study was to examine the effect of isoprene-derived SOA on inflammation-associated gene expression in human lung cells using a direct deposition exposure method. We assessed altered expression of inflammation-related genes in human bronchial epithelial cells (BEAS-2B) exposed to isoprene-derived SOA generated in an outdoor chamber facility. Measurements of gene expression of known inflammatory biomarkers interleukin 8 ( IL-8 ) and cyclooxygenase 2 ( COX-2 ) in exposed cells, together with complementary chemical measurements, showed that a dose of 0.067 µg cm −2 of SOA from isoprene photooxidation leads to statistically significant increases in IL-8 and COX-2 mRNA levels. Resuspension exposures using aerosol filter extracts corroborated these findings, supporting the conclusion that isoprene-derived SOA constituents induce the observed changes in mRNA levels. The present study is an attempt to examine the early biological responses of isoprene SOA exposure in human lung cells. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://acp.copernicus.org/articles/16/14079/2016/ |
| DOI: | 10.5194/acp-16-14079-2016 |
| الاتاحة: | https://doi.org/10.5194/acp-16-14079-2016 https://acp.copernicus.org/articles/16/14079/2016/ |
| رقم الانضمام: | edsbas.D0E726EF |
| قاعدة البيانات: | BASE |
| DOI: | 10.5194/acp-16-14079-2016 |
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