Academic Journal
Separation and quantification of the urinary enantiomers of 2-hydroxyglutaric acid by capillary electrophoresis with capacitively coupled contactless conductivity detection: Application to the diagnosis of D- and L-2-hydroxyglutaric aciduria
| العنوان: | Separation and quantification of the urinary enantiomers of 2-hydroxyglutaric acid by capillary electrophoresis with capacitively coupled contactless conductivity detection: Application to the diagnosis of D- and L-2-hydroxyglutaric aciduria |
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| المؤلفون: | Cansever, M. Serif, Oztekin, Nevin, Kiykim, Ertugrul, Zuebarioglu, Tanyel, Zeybek, Ayse Cigdem Aktuglu |
| بيانات النشر: | Wiley-V C H Verlag Gmbh |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | 2-hydroxyglutaric aciduria, chirality, enantioseparation, metabolic disorder, urine, Tandem Mass-Spectrometry, Lactic-Acid, Organic-Acids, Chromatography, Assay, Tool |
| الوصف: | 2-hydroxyglutaric aciduria is an inherited neurometabolic disorder with two major types: D-2-hydroxyglutaric aciduria and L-2-hydroxyglutaric aciduria. An easy and fast capillary electrophoresis system combined with a capacitively coupled contactless conductivity detection method was developed for the enantioseparation and determination of D- and L-2-hydroxyglutaric acid in urine. D- and L-2-hydroxyglutaric acids were separated using vancomycin as the chiral selector. The optimal separation conditions for enantiomers were achieved by the use of a buffer containing 50 mM 4-(N-morpholino) butane sulfonic acid solution (pH 6.5), an electroosmotic flow modifier (0.001% [w/v] polybrene), and 30 mM vancomycin as chiral selector. The analysis time was 6 min under optimal conditions. The optimized and validated method was successfully implemented for quantifying D- and L-2-hydroxyglutaric aciduria in patients' urine, without any pretreatment step. The linearity of the method was determined to be in the range of 2-100 mg/L for D- and L-2-hydroxyglutaric acid in urine. The precision (relative standard deviation%) was obtained at about 7%. For D- and L-2-hydroxyglutaric acids, the limits of detection were 0.567 and 0.497 mg/L, respectively. ; Research Foundation of Istanbul Technical University [TGA-2019-42232] ; ACKNOWLEDGMENTS The financial support for this research was provided by the Research Foundation of Istanbul Technical University (Project no: TGA-2019-42232). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1615-9306 1615-9314 |
| Relation: | Journal of Separation Science; Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.1002/jssc.202300145; https://hdl.handle.net/20.500.12831/18186; 46; 16; WOS:000998798600001; 2-s2.0-85161414997 |
| DOI: | 10.1002/jssc.202300145 |
| الاتاحة: | https://doi.org/10.1002/jssc.202300145 https://hdl.handle.net/20.500.12831/18186 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.D03741F3 |
| قاعدة البيانات: | BASE |
| تدمد: | 16159306 16159314 |
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| DOI: | 10.1002/jssc.202300145 |