Academic Journal
Expansion of functional personalized cell systems with specific transgene combinations
| العنوان: | Expansion of functional personalized cell systems with specific transgene combinations |
|---|---|
| المؤلفون: | Lipps, Christoph, Klein, Franziska, Wahlicht, Tom, Seiffert, Virginia, Butueva, Milada, Zauers, Jeannette, Truschel, Theresa, Luckner, Martin, Köster, Mario, MacLeod, Roderick, Pezoldt, Jörn, Hühn, Jochen, Yuan, Qinggong, Müller, Peter Paul, Kempf, Henning, Zweigerdt, Robert, Dittrich-Breiholz, Oliver, Pufe, Thomas, Riancho Moral, José Antonio, Sañudo Campo, María Carolina |
| المساهمون: | Universidad de Cantabria |
| المصدر: | Nature Communications 2018; 9(1):994 |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2018 |
| المجموعة: | Universidad de Cantabria: UCrea |
| الوصف: | Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development. ; Acknowledgements: This work was supported by grants from the Niedersächsisches Ministerium für Wissenschaft und Kultur (80029155), the German Ministry for Economic Affairs and Energy (IGF 16153 N) and the German Ministry for Research and Education (FKZ 0316037). The authors further acknowledge funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) for the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy) and WI2648/3-1. Further, T. Wahlicht, F. Klein M. Butueva, and C. Lipps wish to acknowledge the support by the HZI GradSchool and the PhD program Regenerative Sciences within the Hannover Biomedical Research School (HBRS). J.A. Riancho and C. Sañudo have support from Instituto de Salud Carlos III/FEDER funds (PI 12/615) and from IDIVAL. B. Opalka thanks Sana Mohamad for technical assistance und B.Opalka, J.R.Göthert, and V.Rebmann thank Ulrich Dührsen and Peter Horn for ongoing support. R.A.F. MacLeod acknowledges support of the Leukemia and Lymphoma Society of America and the expert technical help of Karen Kaufmann. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2041-1723 |
| Relation: | https://dx.doi.org/10.1038/s41467-018-03408-4; http://hdl.handle.net/10902/15667 |
| DOI: | 10.1038/s41467-018-03408-4 |
| الاتاحة: | http://hdl.handle.net/10902/15667 https://doi.org/10.1038/s41467-018-03408-4 |
| Rights: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; openAccess |
| رقم الانضمام: | edsbas.D0314374 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20411723 |
|---|---|
| DOI: | 10.1038/s41467-018-03408-4 |