التفاصيل البيبلوغرافية
| العنوان: |
Astaxanthin Exerts Immunomodulatory Effect by Regulating SDH-HIF-1α Axis and Reprogramming Mitochondrial Metabolism in LPS-Stimulated RAW264.7 Cells |
| المؤلفون: |
Sun, Luchuanyang, Kim, Sangeun, Mori, Ryoichi, Miyaji, Nobuyuki, Nikawa, Takeshi, Hirasaka, Katsuya |
| بيانات النشر: |
MDPI |
| سنة النشر: |
2024 |
| المجموعة: |
Tokushima University Institutional Repository / 徳島大学機関リポジトリ |
| مصطلحات موضوعية: |
astaxanthin, mitochondrial ROS, mitochondrial energy metabolism, mitochondrial succinate dehydrogenase, hypoxia-inducible factor-1α, interleukin-1β |
| الوصف: |
Astaxanthin (AX) is a carotenoid that exerts potent antioxidant activity and acts in cell membranes and mitochondria, which consist of the bilayer molecules. Targeting mitochondria to ameliorate inflammatory diseases by regulating mitochondrial metabolism has become possible and topical. Although AX has been shown to have anti-inflammatory effects in various cells, the mechanisms are quite different. In particular, the role of AX on mitochondrial metabolism in macrophages is still unknown. In this study, we investigated the effect of AX on mitochondria-mediated inflammation and its mechanisms in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. AX attenuated the mitochondrial O2− production and maintained the mitochondrial membrane potential, implying that AX preserved mitochondrial homeostasis to avoid LPS stimulation-induced mitochondrial dysfunction. Additionally, AX prevented the decrease in mitochondrial complexes I, II, and III, which were caused by LPS stimulation. Especially, AX inhibited the reduction in mitochondrial succinate dehydrogenase (SDH; complex II) activity and upregulated the protein and mRNA level of SDH complex, subunit B. Furthermore, AX blocked the IL-1β expression by regulating the SDH-HIF-1α axis and suppressed the energy shift from an OXPHOS phenotype to a glycolysis phenotype. These findings revealed important effects of AX on mitochondrial enzymes as well as on mitochondrial energy metabolism in the immune response. In addition, these raised the possibility that AX plays an important role in other diseases caused by SDH mutation and metabolic disorders. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| تدمد: |
16603397 |
| Relation: |
http://repo.lib.tokushima-u.ac.jp/files/public/11/118918/20240118133822101951/md_20_11_660.pdf; http://repo.lib.tokushima-u.ac.jp/118918 |
| الاتاحة: |
http://repo.lib.tokushima-u.ac.jp/118918 |
| Rights: |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| رقم الانضمام: |
edsbas.CFD36A54 |
| قاعدة البيانات: |
BASE |
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