Academic Journal
Cilostazol Suppresses IL-23 Production in Human Dendritic Cells via an AMPK-Dependent Pathway
| العنوان: | Cilostazol Suppresses IL-23 Production in Human Dendritic Cells via an AMPK-Dependent Pathway |
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| المؤلفون: | Shi, Quanxing, Yin, Zhao, Liu, Peilin, Zhao, Bei, Zhang, Zhong, Mao, Shuai, Wei, Tengfei, Rao, Mengmeng, Zhang, Liguo, Wang, Shouli |
| المصدر: | Cellular Physiology and Biochemistry ; volume 40, issue 3-4, page 499-508 ; ISSN 1015-8987 1421-9778 |
| بيانات النشر: | S. Karger AG |
| سنة النشر: | 2016 |
| الوصف: | Background/Aims: Cilostazol has been previously demonstrated to inhibit IL-23 production in human synovial macrophages via a RhoA/ROCK-dependent pathway. However, whether cilostazol affects IL-23 production in human dendritic cells remains largely unknown. The present study was designed to investigate this question and elucidate the possible underlying mechanisms. Methods: Human monocyte-derived dendritic cells (mo-DCs) were pretreated with or without cilostazol and then incubated with zymosan. Enzyme-linked immunosorbent assay (ELISA) and real time PCR analyses were used to measure IL-23 protein expression and RNA levels, respectively, whereas Western blotting was used to measure the expression and phosphorylation level of AMPK. Results: Our results demonstrated that cilostazol suppressed zymosan-induced IL-23 protein production in a concentration dependent manner without affecting dendritic cell viability. In addition, it was found that cilostazol suppressed the expression of the p19 and p40 subunits of IL-23. Moreover, cilostazol mimicked the effect of the AMPK agonist A-769662, as demonstrated by the fact that IL-23 production was also inhibited by A-769662, and the effect of cilostazol on IL-23 production was blocked by the AMPK antagonist Compound C. More importantly, Western blotting demonstrated that cilostazol led to an increased phosphorylation of AMPK. Conclusion: Collectively, our data suggest that cilostazol inhibits the production of IL-23 in human mo-DCs, potentially via the activation of AMPK. This suggests that cilostazol could be an effective anti-inflammatory agent in IL-23- and dendritic cell-related diseases. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1159/000452564 |
| الاتاحة: | http://dx.doi.org/10.1159/000452564 https://www.karger.com/Article/Pdf/452564 |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ ; https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.CF8BDDF4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1159/000452564 |
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