Academic Journal
Calcium-evoked dendritic exocytosis in cultured hippocampal neurons. Part I: trans-Golgi networkderived organelles undergo regulated exocytosis. J Neurosci 18:6803–6813
| العنوان: | Calcium-evoked dendritic exocytosis in cultured hippocampal neurons. Part I: trans-Golgi networkderived organelles undergo regulated exocytosis. J Neurosci 18:6803–6813 |
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| المؤلفون: | Mirjana Maletic-savatic, Thillai Koothan, Roberto Malinow |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://malinowlab.cshl.edu/Malinow Images/PDF papers/Maletic II 1998.pdf. |
| سنة النشر: | 1998 |
| المجموعة: | CiteSeerX |
| الوصف: | cultured hippocampal neurons, is a novel mechanism that could play a role in synaptic plasticity. A number of forms of neuronal plasticity are thought to be mediated by calcium/ calmodulin-dependent protein kinase II (CaMKII). Here, we investigate the role of CaMKII in CEDE. We find that the developmental time course of CEDE parallels the expression of �CaMKII, a dominant subunit of CaMKII. An inhibitor of this enzyme, KN-62, blocks CEDE. Furthermore, 7 d in vitro neurons (which normally do not express �CaMKII nor show CEDE) can undergo CEDE when infected with a recombinant virus producing �CaMKII. Expression of a constitutively active CaMKII produces dendritic exocytosis in the absence of calcium stimulus, and this exocytosis is blocked by nocodazole, an inhibitor of microtubule polymerization that also blocks CEDE. These results indicate that CEDE is mediated by the activation of CaMKII, consistent with the view that CEDE plays a role in synaptic plasticity. Key words: FM1-43; exocytosis; dendrites; pyramidal neurons; hippocampal cultures; CaMKII; KN-62; immunocytochemistry; vaccinia virus Calcium-evoked dendritic exocytosis (CEDE) is a novel process that might be involved in the regulated delivery of dendritic and postsynaptic membrane-bound proteins. The essential feature of CEDE is its triggering by calcium. In principal projection neurons a major constituent of synapses is the calcium-sensitive enzyme calcium/calmodulin-dependent protein kinase II (CaMKII) |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.318.8119; http://malinowlab.cshl.edu/Malinow Images/PDF papers/Maletic II 1998.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.318.8119 http://malinowlab.cshl.edu/Malinow Images/PDF papers/Maletic II 1998.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.CF5EADE1 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |