التفاصيل البيبلوغرافية
| العنوان: |
Cooperation among c-subunits of FoF1-ATP synthase in rotation-coupled proton translocation |
| المؤلفون: |
Mitome, Noriyo, Kubo, Shintaroh, Ohta, Sumie, Takashima, Hikaru, Shigefuji, Yuto, Niina, Toru, Takada, Shoji |
| المساهمون: |
久保, 進太郎, 新稲, 亮, 高田, 彰二 |
| بيانات النشر: |
eLife Sciences Publications, Ltd |
| سنة النشر: |
2022 |
| المجموعة: |
Kyoto University Research Information Repository (KURENAI) / 京都大学学術情報リポジトリ |
| مصطلحات موضوعية: |
Research Article, Biochemistry and Chemical Biology, FoF1-ATP synthase, single-chain c-ring, proton uptake, molecular simulations |
| الوصف: |
In F₀F₁-ATP synthase, proton translocation through F₀ drives rotation of the c-subunit oligomeric ring relative to the a-subunit. Recent studies suggest that in each step of the rotation, key glutamic acid residues in different c-subunits contribute to proton release to and proton uptake from the a-subunit. However, no studies have demonstrated cooperativity among c-subunits toward F₀F₁-ATP synthase activity. Here, we addressed this using Bacillus PS3 ATP synthase harboring a c-ring with various combinations of wild-type and cE56D, enabled by genetically fused single-chain c-ring. ATP synthesis and proton pump activities were decreased by a single cE56D mutation and further decreased by double cE56D mutations. Moreover, activity further decreased as the two mutation sites were separated, indicating cooperation among c-subunits. Similar results were obtained for proton transfer-coupled molecular simulations. The simulations revealed that prolonged proton uptake in mutated c-subunits is shared between two c-subunits, explaining the cooperation observed in biochemical assays. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| تدمد: |
2050-084X |
| Relation: |
http://hdl.handle.net/2433/276322; eLife; 11; e69096 |
| الاتاحة: |
http://hdl.handle.net/2433/276322 |
| Rights: |
© 2022, Mitome et al. ; This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.CF3D118E |
| قاعدة البيانات: |
BASE |
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