Report
Microtubule plus-end regulation by centriolar cap proteins
| العنوان: | Microtubule plus-end regulation by centriolar cap proteins |
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| المؤلفون: | Ogunmolu, Funso, E, Moradi, Shoeib, Volkov, Vladimir, A, van Hoorn, Chris, Wu, Jingchao, Andrea, Nemo, Hua, Shasha, Jiang, Kai, Vakonakis, Ioannis, Potočnjak, Mia, Herzog, Franz, Gigant, Benoît, Gudimchuk, Nikita, Stecker, Kelly, E, Dogterom, Marileen, Steinmetz, Michel, O, Akhmanova, Anna |
| المساهمون: | Department of Biology, Faculty of Sciences, Utrecht University, Paul Scherrer Institute (PSI), Ludwig Maximilian University Munich = Ludwig Maximilians Universität München (LMU), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | https://hal.science/hal-03829938 ; 2022. |
| بيانات النشر: | CCSD |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| الوصف: | Centrioles are microtubule-based organelles required for the formation of centrosomes and cilia. Centriolar microtubules, unlike their cytosolic counterparts, grow very slowly and are very stable. The complex of centriolar proteins CP110 and CEP97 forms a cap that stabilizes the distal centriole end and prevents its over-elongation. Here, we used in vitro reconstitution assays to show that whereas CEP97 does not interact with microtubules directly, CP110 specifically binds microtubule plus ends, potently blocks their growth and induces microtubule pausing. Cryo-electron tomography indicated that CP110 binds to the luminal side of microtubule plus ends and reduces protofilament peeling. Furthermore, CP110 directly interacts with another centriole biogenesis factor, CPAP/SAS- 4, which tracks growing microtubule plus ends, slows down their growth and prevents catastrophes. CP110 and CPAP synergize in inhibiting plus-end growth, and this synergy depends on their direct binding. Together, our data reveal a molecular mechanism controlling centriolar microtubule plus- end dynamics and centriole biogenesis. |
| نوع الوثيقة: | report |
| اللغة: | English |
| Relation: | BIORXIV: 2021.12.29.474442 |
| DOI: | 10.1101/2021.12.29.474442 |
| الاتاحة: | https://hal.science/hal-03829938 https://hal.science/hal-03829938v1/document https://hal.science/hal-03829938v1/file/2021.12.29.474442v1.full.pdf https://doi.org/10.1101/2021.12.29.474442 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.CEBBE1F9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2021.12.29.474442 |
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