التفاصيل البيبلوغرافية
| العنوان: |
Image1_Meclozine Attenuates the MARK Pathway in Mammalian Chondrocytes and Ameliorates FGF2-Induced Bone Hyperossification in Larval Zebrafish.TIF |
| المؤلفون: |
Genta Takemoto (10584615), Masaki Matsushita (492573), Takaaki Okamoto (11957762), Toshinari Ito (11957765), Yuki Matsuura (757718), Chieko Takashima (11957768), Toyofumi Fengshi Chen-Yoshikawa (9647623), Hiromichi Ebi (412406), Shiro Imagama (426734), Hiroshi Kitoh (492574), Kinji Ohno (157798), Yasuyuki Hosono (218390) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Cell Biology, Marine Biology, Cell Development, Proliferation and Death, Cell Metabolism, Cell Neurochemistry, Cellular Interactions (incl. Adhesion, Matrix, Cell Wall), FGFR3, achondroplasia, meclozine, zebrafish, bone |
| الوصف: |
Meclozine has been developed as an inhibitor of fibroblast growth factor receptor 3 (FGFR3) to treat achondroplasia (ACH). Extracellular signal regulated kinase (ERK) phosphorylation was attenuated by meclozine in FGF2-treated chondrocyte cell line, but the site of its action has not been elucidated. Although orally administered meclozine promoted longitudinal bone growth in a mouse model of ACH, its effect on craniofacial bone development during the early stage remains unknown. Herein, RNA-sequencing analysis was performed using murine chondrocytes from FGF2-treated cultured tibiae, which was significantly elongated by meclozine treatment. Gene set enrichment analysis demonstrated that FGF2 significantly increased the enrichment score of mitogen-activated protein kinase (MAPK) family signaling cascades in chondrocytes; however, meclozine reduced this enrichment. Next, we administered meclozine to FGF2-treated larval zebrafish from 8 h post-fertilization (hpf). We observed that FGF2 significantly increased the number of ossified vertebrae in larval zebrafish at 7 days post-fertilization (dpf), while meclozine delayed vertebral ossification in FGF2-induced zebrafish. Meclozine also reversed the FGF2-induced upregulation of ossified craniofacial bone area, including ceratohyal, hyomandibular, and quadrate. The current study provided additional evidence regarding the inhibitory effect of meclozine on the FGF2-induced upregulation of MAPK signaling in chondrocytes and FGF2-induced development of craniofacial and vertebral bones. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Image1_Meclozine_Attenuates_the_MARK_Pathway_in_Mammalian_Chondrocytes_and_Ameliorates_FGF2-Induced_Bone_Hyperossification_in_Larval_Zebrafish_TIF/18584585 |
| DOI: |
10.3389/fcell.2021.694018.s001 |
| الاتاحة: |
https://doi.org/10.3389/fcell.2021.694018.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.CE583D68 |
| قاعدة البيانات: |
BASE |