Academic Journal
A single residue in a novel ADP-ribosyl cyclase controls production of the calcium-mobilizing messengers cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate.
| العنوان: | A single residue in a novel ADP-ribosyl cyclase controls production of the calcium-mobilizing messengers cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate. |
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| المؤلفون: | Ramakrishnan, Latha, Muller-Steffner, Hélène, Bosc, Christophe, Vacquier, Victor, D., Schuber, Francis, Moutin, Marie-Jo, Dale, Leslie, Patel, Sandip |
| المساهمون: | Department of Cell and Developmental Biology, University College of London London (UCL), Faculté de Pharmacie, Groupe Physiopathologie du Cytosquelette (GPC), Institut National de la Santé et de la Recherche Médicale (INSERM)- GIN Grenoble Institut des Neurosciences (GIN), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Marine Biology Research Division, Scripps Institution of Oceanography (SIO - UC San Diego), University of California San Diego (UC San Diego), University of California (UC)-University of California (UC)-University of California San Diego (UC San Diego), University of California (UC)-University of California (UC), Grants BB/D018110/1 and BB/G013721/1 from the Biotechnology and Biological Sciences Research Council (to S.P.). PhD studentship from the Medical Research Council (to L.R.). |
| المصدر: | ISSN: 0021-9258. |
| بيانات النشر: | HAL CCSD American Society for Biochemistry and Molecular Biology |
| سنة النشر: | 2010 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| الوصف: | International audience ; Cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate are ubiquitous calcium-mobilizing messengers produced by the same family of multifunctional enzymes, the ADP-ribosyl cyclases. Not all ADP-ribosyl cyclases have been identified, and how production of different messengers is achieved is incompletely understood. Here, we report the cloning and characterization of a novel ADP-ribosyl cyclase (SpARC4) from the sea urchin, a key model organism for the study of calcium-signaling pathways. Like several other members of the ADP-ribosyl cyclase superfamily, SpARC4 is a glycoprotein targeted to the plasma membrane via a glycosylphosphatidylinositol anchor. However, unlike most other members, SpARC4 shows a remarkable preference for producing cyclic ADP-ribose over nicotinic acid adenine dinucleotide phosphate. Mutation of a single residue (tyrosine 142) within a noncanonical active site reversed this striking preference. Our data highlight further diversification of this unusual enzyme family, provide mechanistic insight into multifunctionality, and suggest that different ADP-ribosyl cyclases are fine-tuned to produce specific calcium-mobilizing messengers. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/20385553; inserm-00497582; https://inserm.hal.science/inserm-00497582; https://inserm.hal.science/inserm-00497582/document; https://inserm.hal.science/inserm-00497582/file/inserm-00497582_edited.pdf; https://inserm.hal.science/inserm-00497582/file/Ramakrishnan_-A_single_residue_in_a_novel_ADP-ribosyl_cyclase_controls_production_of_the_calcium_mobilizing_messengers_2010.pdf; PUBMED: 20385553 |
| DOI: | 10.1074/jbc.M110.105312 |
| الاتاحة: | https://inserm.hal.science/inserm-00497582 https://inserm.hal.science/inserm-00497582/document https://inserm.hal.science/inserm-00497582/file/inserm-00497582_edited.pdf https://inserm.hal.science/inserm-00497582/file/Ramakrishnan_-A_single_residue_in_a_novel_ADP-ribosyl_cyclase_controls_production_of_the_calcium_mobilizing_messengers_2010.pdf https://doi.org/10.1074/jbc.M110.105312 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.CE4D447B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1074/jbc.M110.105312 |
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