التفاصيل البيبلوغرافية
| العنوان: |
Combining random microseed matrix screening and the magic triangle for the efficient structure solution of a potential lysin from bacteriophage P68 |
| المؤلفون: |
Jia Quyen Truong, Santosh Panjikar, Linda Shearwin-Whyatt, John Bruning, Keith Shearwin |
| سنة النشر: |
2019 |
| مصطلحات موضوعية: |
Structural biology (incl. macromolecular modelling), I3C, crystallography, lysin, magic triangle, phasing, random microseed matrix screening, seeding |
| الوصف: |
Two commonly encountered bottlenecks in the structure determination of a protein by X-ray crystallography are screening for conditions that give high-quality crystals and, in the case of novel structures, finding derivatization conditions for experimental phasing. In this study, the phasing molecule 5-amino-2,4,6-triiodoisophthalic acid (I3C) was added to a random microseed matrix screen to generate high-quality crystals derivatized with I3C in a single optimization experiment. I3C, often referred to as the magic triangle, contains an aromatic ring scaffold with three bound I atoms. This approach was applied to efficiently phase the structures of hen egg-white lysozyme and the N-terminal domain of the Orf11 protein from Staphylococcus phage P68 (Orf11 NTD) using SAD phasing. The structure of Orf11 NTD suggests that it may play a role as a virion-associated lysin or endolysin. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
10779/rmit.27557136.v1; https://figshare.com/articles/journal_contribution/Combining_random_microseed_matrix_screening_and_the_magic_triangle_for_the_efficient_structure_solution_of_a_potential_lysin_from_bacteriophage_P68/27557136 |
| الاتاحة: |
https://figshare.com/articles/journal_contribution/Combining_random_microseed_matrix_screening_and_the_magic_triangle_for_the_efficient_structure_solution_of_a_potential_lysin_from_bacteriophage_P68/27557136 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.CE10C2F7 |
| قاعدة البيانات: |
BASE |