Academic Journal
Kanamycin Uptake into Escherichia coli Is Facilitated by OmpF and OmpC Porin Channels Located in the Outer Membrane
| العنوان: | Kanamycin Uptake into Escherichia coli Is Facilitated by OmpF and OmpC Porin Channels Located in the Outer Membrane |
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| المؤلفون: | Bafna, Jayesh Arun, Sans-Serramitjana, Eulàlia, Acosta-Gutiérrez, Silvia, Bodrenko, Igor V, Hörömpöli, Daniel, Berscheid, Anne, Brötz-Oesterhelt, Heike, Winterhalter, Mathias, Ceccarelli, Matteo |
| المساهمون: | Bafna, Jayesh Arun, Sans-Serramitjana, Eulàlia, Acosta-Gutiérrez, Silvia, Bodrenko, Igor V, Hörömpöli, Daniel, Berscheid, Anne, Brötz-Oesterhelt, Heike, Winterhalter, Mathia, Ceccarelli, Matteo |
| سنة النشر: | 2020 |
| المجموعة: | Università degli Studi di Cagliari: UNICA IRIS |
| مصطلحات موضوعية: | E. , coli, OmpC, OmpF, OmpN, Bacterial porin, Optimal transport |
| الوصف: | Despite decades of therapeutic application of aminoglycosides, it is still a matter of debate if porins contribute to the translocation of the antibiotics across the bacterial outer membrane. Here, we quantified the uptake of kanamycin across the major porin channels OmpF and OmpC present in the outer membrane of Escherichia coli. Our analysis revealed that, despite its relatively large size, about 10-20 kanamycin molecules per second permeate through OmpF and OmpC under a 10 μM concentration gradient, whereas OmpN does not allow the passage. Molecular simulations elucidate the uptake mechanism of kanamycin through these porins. Whole-cell studies with a defined set of E.coli porin mutants provide evidence that translocation of kanamycin via porins is relevant for antibiotic potency. The values are discussed with respect to other antibiotics. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/32369342; info:eu-repo/semantics/altIdentifier/wos/WOS:000595290900035; volume:6; issue:7; firstpage:1855; lastpage:1865; numberofpages:11; journal:ACS INFECTIOUS DISEASES; http://hdl.handle.net/11584/293139; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85088486911 |
| DOI: | 10.1021/acsinfecdis.0c00102 |
| الاتاحة: | http://hdl.handle.net/11584/293139 https://doi.org/10.1021/acsinfecdis.0c00102 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.CD951262 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acsinfecdis.0c00102 |
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