Academic Journal
Comparison of superovulatory responses to a standardized hormonal superstimulation protocol among three indigenous breeds of sheep in Brazil
| العنوان: | Comparison of superovulatory responses to a standardized hormonal superstimulation protocol among three indigenous breeds of sheep in Brazil |
|---|---|
| المؤلفون: | Pupin, Maria Amélia Ferrão, Oliveira, Maria Emilia Franco, Vergani, Gabriel Brun, Lima, Monalisa Sousa Dias, Silva, Kleibe de Moraes, Monteiro, Alexandre Weick Uchôa, Ramos, Alexandre Floriani, Batista, Ribrio Ivan Tavares Pereira, Souza-Fabjan, Joanna Maria Gonçalves, Bartlewski, Pawel Mieczyslaw, Fonseca, Jeferson Ferreira |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| سنة النشر: | 2022 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | Color Doppler, Corpora lutea, Ewe, Ovarian antral follicles, Ovarian blood perfusion, Superovulation |
| الوصف: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) ; Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) ; FAPEMIG: 00201-17 ; This study examined the differences in ovarian responses and in vivo embryo production in three naturalized Brazilian sheep breeds (Morada Nova, Santa Inês, and Somalis Brasileira). Thirty cycling, multiparous ewes (n = 10/breed) received intravaginal devices containing 0.33 g of progesterone (CIDR®) for 9 days and the twice-daily superovulatory treatment with 133 mg of porcine follicle-stimulating hormone (six decreasing doses) started on Day 7 (Day 0 PM = CIDR® insertion). On Days 11 (36 h after CIDR® removal) and 15 (12 h before non-surgical embryo recovery performed seven days after CIDR® removal), all ewes were examined using B-mode and Doppler ultrasonography. The number of medium-sized ovarian follicles (4.0–5.99 mm) on Day 11 was greater (P = 0.003) in Santa Inês and Somalis Brasileira compared with Morada Nova ewes. Small antral follicle numbers (2.0–3.99 mm in diameter) on Day 11 were directly related to ovulatory responses in all three breeds of superovulated ewes. The number of corpora lutea on Day 15 was greater (P < 0.0001) in Santa Inês, followed by Somalis Brasileira and Morada Nova ewes; however, the number of viable embryos and viability rate did not differ (P > 0.05) among the three genotypes of ewes studied. The total ovarian area (TA), color Doppler area (DA), and DA/TA × 100% increased from Day 11 to Day 15 in all breeds, and they were lowest (P < 0.001) in superovulated Morada Nova sheep. Significant positive correlations among ovarian antral follicle numbers in different size classes (Day 11) and ovulatory responses were recorded in all breeds, but associations between the ovarian blood perfusion and superovulatory outcomes were restricted to the Morada Nova and Santa Inês genotypes. In summary, ovarian follicle numbers and blood flow indices differed among the three naturalized Brazilian ewes, but no significant differences ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0921-4488 |
| Relation: | Small Ruminant Research; http://dx.doi.org/10.1016/j.smallrumres.2022.106703; Small Ruminant Research, v. 211.; http://hdl.handle.net/11449/240001; 2-s2.0-85129484576 |
| DOI: | 10.1016/j.smallrumres.2022.106703 |
| الاتاحة: | http://hdl.handle.net/11449/240001 https://doi.org/10.1016/j.smallrumres.2022.106703 |
| رقم الانضمام: | edsbas.CD00F4AD |
| قاعدة البيانات: | BASE |
| تدمد: | 09214488 |
|---|---|
| DOI: | 10.1016/j.smallrumres.2022.106703 |