Academic Journal
Use of Pinus sylvestris L. (Pinaceae), Origanum vulgare L. (Lamiaceae), and Thymus vulgaris L. (Lamiaceae) essential oils and their main components to enhance itraconazole activity against azole susceptible/not-susceptible Cryptococcus neoformans strains
| العنوان: | Use of Pinus sylvestris L. (Pinaceae), Origanum vulgare L. (Lamiaceae), and Thymus vulgaris L. (Lamiaceae) essential oils and their main components to enhance itraconazole activity against azole susceptible/not-susceptible Cryptococcus neoformans strains |
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| المؤلفون: | Scalas, Daniela, Mandras, Narcisa, Roana, Janira, Tardugno, Roberta, Cuffini, Anna Maria, Ghisetti, Valeria, Benvenuti, Stefania, Tullio, Vivian |
| المساهمون: | Scalas, Daniela, Mandras, Narcisa, Roana, Janira, Tardugno, Roberta, Cuffini, Anna Maria, Ghisetti, Valeria, Benvenuti, Stefania, Tullio, Vivian |
| سنة النشر: | 2018 |
| المجموعة: | Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) |
| مصطلحات موضوعية: | Keywords: Cryptococcus neoformans, Essential oils, Itraconazole, Antifungal activity, Combinatorial interaction |
| الوصف: | Background: Cryptococcal infections, besides being a problem for immunocompromised patients, are occasionally being a problem for immunocompetent patients. In addition, the lower susceptibility of this yeast to azoles is a growing problem in health care. To date, there are very few molecules with any activity towards Cryptococcus neoformans, leading to heightened interest in finding new alternatives or adjuvants to conventional drugs for the treatment of mycosis caused by this yeast. Since the essential oils (EOs) are considered as a potential rich source of bioactive antimicrobial compounds, we evaluated the antifungal activity of Origanum vulgare (oregano), Pinus sylvestris (pine), and Thymus vulgaris (thyme red) EOs, and their components (α-pinene, carvacrol, thymol) compared with fluconazole, itraconazole, and voriconazole, against C.neoformans clinical strains. Then, we investigated the effect of EOs and components in combination with itraconazole. Methods: EO composition was analysed by Gas chromatography-mass spectrometry (GC-MS). A broth microdilution method was used to evaluate the susceptibility of C.neoformans to azoles, EOs and components. Checkerboard tests, isobolograms and time-kill assays were carried out for combination studies. Results: C.neoformans isolates were susceptible to azoles, while one C.neoformans exhibited a reduced susceptibility to all test edazole drugs. All EOs exerted a good inhibitory activity against all C.neoformans strains. Pine EO was the most effective. Among components, thymol exerted the most remark able activity. By checkerboard testing and isobolographic analysis, combinations of itraconazole with oregano, pine, or thyme EOs, and carvacrol were found to be synergistic (FICI ≤ 0.5) against azole susceptible C.neoformans. Regarding the azole not susceptible C.neoformans strain, the synergistic effect with itraconazole was observed with thyme EO (chemotype: thymol 26.52%; carvacrol 7.85%), and carvacrol. Time-kill assays confirmed the synergistic effects of ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/29724221; info:eu-repo/semantics/altIdentifier/wos/WOS:000431322200002; volume:18; issue:1; firstpage:1; lastpage:13; journal:BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE; http://hdl.handle.net/11380/1160818; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85046459007 |
| DOI: | 10.1186/s12906-018-2219-4 |
| الاتاحة: | http://hdl.handle.net/11380/1160818 https://doi.org/10.1186/s12906-018-2219-4 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.CCF02DD6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12906-018-2219-4 |
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