Academic Journal
Immunoglobulin G elution in protein A chromatography employing the method of chromatofocusing for reducing the co‐elution of impurities
| العنوان: | Immunoglobulin G elution in protein A chromatography employing the method of chromatofocusing for reducing the co‐elution of impurities |
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| المؤلفون: | Pinto, Nuno D.S., Uplekar, Shaunak D., Moreira, Antonio R., Rao, Govind, Frey, Douglas D. |
| المساهمون: | National Science Foundation |
| المصدر: | Biotechnology and Bioengineering ; volume 114, issue 1, page 154-162 ; ISSN 0006-3592 1097-0290 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2016 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Purification processes for monoclonal Immunoglobulin G (IgG) typically employ protein A chromatography as a capture step to remove most of the impurities. One major concern of the post‐protein A chromatography processes is the co‐elution of some of the host cell proteins (HCPs) with IgG in the capture step. In this work, a novel method for IgG elution in protein A chromatography that reduces the co‐elution of HCPs is presented where a two‐step pH gradient is self‐formed inside a protein A chromatography column. The complexities involved in using an internally produced pH gradient in a protein A chromatography column employing adsorbed buffering species are discussed though equation‐based modeling. Under the conditions employed, ELISA assays show a 60% reduction in the HCPs co‐eluting with the IgG fraction when using the method as compared to conventional protein A elution without affecting the IgG yield. Evidence is also obtained which indicates that the amount of leached protein A present in free solution in the purified product is reduced by the new method. Biotechnol. Bioeng. 2017;114: 154–162. © 2016 Wiley Periodicals, Inc. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/bit.26053 |
| الاتاحة: | http://dx.doi.org/10.1002/bit.26053 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.26053 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.26053 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/bit.26053 https://onlinelibrary.wiley.com/doi/am-pdf/10.1002/bit.26053 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#am ; http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.CCDC25E2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/bit.26053 |
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