Conference
The Intracellular Ion flux mediated by TMEM176A and TMEM176B is involved in the regulation of Dendritic Cell Functions
| العنوان: | The Intracellular Ion flux mediated by TMEM176A and TMEM176B is involved in the regulation of Dendritic Cell Functions |
|---|---|
| المؤلفون: | Lancien, Mélanie, Bienvenu-Louvet, Géraldine, Guéno, Lucile, Moreau, Aurélie, Salle, Sonia, Merieau, Emmanuel, Bouchet-Delbos, Laurence, Coulon, Flora, Boncompain, Gaëlle, Perez, Franck, Cuturi, Maria, Cristina, Louvet, Cédric |
| المساهمون: | Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes), Département de Structure Subcellulaire et Dynamique Cellulaire Paris, Université Paris Dauphine-PSL, Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut Curie Paris -Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Focis ; https://inserm.hal.science/inserm-02158907 ; Focis, Jun 2019, Boston, United States |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2019 |
| المجموعة: | Université de Nantes: HAL-UNIV-NANTES |
| مصطلحات موضوعية: | [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| جغرافية الموضوع: | Boston, United States |
| Time: | Boston, United States |
| الوصف: | International audience ; The regulation of immune cells by intracellular ion channels remains poorly explored. Here we investigated the role of two cation channels encoded by Tmem176a and Tmem176b that are highly expressed in myeloid cells in the immune system. To avoid any functional compensation, we generated double knock-out (DKO) mice, allowing simultaneous deletion of these two highly redundant and coregulated genes using the CRISPR-Cas9 system. The absence of Tmem176a/b significantly impacted antigen processing and presentation to CD4+ T cells in vivo and selectively altered cytokine production by dendritic cells (DCs). Using a novel real-time fluorescence-based system to analyze intracellular trafficking we found that both channels co-localized in highly dynamic post-Golgi vesicles preferentially interacting with, but not accumulating in, acidic organelles. Thus, these results highlight the importance of TMEM176A/B-mediated cation flux for the fine regulation of DC biology. |
| نوع الوثيقة: | conference object still image |
| اللغة: | English |
| Relation: | inserm-02158907; https://inserm.hal.science/inserm-02158907; https://inserm.hal.science/inserm-02158907/document; https://inserm.hal.science/inserm-02158907/file/Poster%20Ecole%20doctorale%20%281%29.pdf |
| الاتاحة: | https://inserm.hal.science/inserm-02158907 https://inserm.hal.science/inserm-02158907/document https://inserm.hal.science/inserm-02158907/file/Poster%20Ecole%20doctorale%20%281%29.pdf |
| رقم الانضمام: | edsbas.CCDA0E0E |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |