Academic Journal
Selective Up‐Regulation of α1‐Chimaerin mRNA in SK‐N‐SH Neuroblastoma Cells by K + ‐Induced Depolarisation
| العنوان: | Selective Up‐Regulation of α1‐Chimaerin mRNA in SK‐N‐SH Neuroblastoma Cells by K + ‐Induced Depolarisation |
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| المؤلفون: | Dong, Jing‐Ming, Lim, Louis |
| المصدر: | European Journal of Biochemistry ; volume 236, issue 3, page 820-826 ; ISSN 0014-2956 1432-1033 |
| بيانات النشر: | Wiley |
| سنة النشر: | 1996 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | The expression of α1‐chimaerin, which encodes a neuron‐specific GTPase‐activating protein for p21rac, is spatially and temporally regulated in vivo . In vitro , expression of the mRNA of both α1‐chimaerin and its alternative spliced form, α2‐chimaerin, was up‐regulated when human neuroblastoma SK‐N‐SH cells underwent neuronal‐type differentiation in a serum‐free medium. KCl‐induced membrane depolarisation also specifically up‐regulated α1‐chimaerin mRNA expression in SK‐N‐SH cells at the transcriptional level. The up‐regulation of α1‐chimaerin expression by membrane depolarisation is not an immediate early event, and occurs 3 h after KCl treatment. It does not require de novo protein synthesis. The increase in calcium influx via the L‐type voltage‐sensitive calcium channel as the result of depolarisation is a key event leading to the up‐regulation of α1‐chimaerin mRNA. α1‐Chimaerin expression was also found to respond positively to the hypertonic osmolarity changes. These results suggest that in vivo expression of α1‐chimaerin, a potential signal transduction molecule, may be regulated by neuronal/synaptic activity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1432-1033.1996.00820.x |
| الاتاحة: | http://dx.doi.org/10.1111/j.1432-1033.1996.00820.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1996.00820.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1996.00820.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.CCAA9707 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1432-1033.1996.00820.x |
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