Academic Journal
Multifaceted N-degron recognition and ubiquitylation by GID/CTLH E3 ligases.
| العنوان: | Multifaceted N-degron recognition and ubiquitylation by GID/CTLH E3 ligases. |
|---|---|
| المؤلفون: | Chrustowicz, J., Sherpa, D., Teyra, J., Loke, M.S., Popowicz, G.M., Basquin, J., Sattler, M., Prabu, J.R., Sidhu, S.S., Schulman, B.A. |
| المصدر: | J. Mol. Biol. 434:167347 (2022) |
| بيانات النشر: | Academic Press Ltd- Elsevier Science Ltd |
| سنة النشر: | 2022 |
| المجموعة: | PuSH - Publikationsserver des Helmholtz Zentrums München |
| مصطلحات موضوعية: | N-degron Pathway, Phage Display, Protein–protein Interaction, Structural Biology, Ubiquitin |
| الوصف: | N-degron E3 ubiquitin ligases recognize specific residues at the N-termini of substrates. Although molecular details of N-degron recognition are known for several E3 ligases, the range of N-terminal motifs that can bind a given E3 substrate binding domain remains unclear. Here, we discovered capacity of Gid4 and Gid10 substrate receptor subunits of yeast “GID”/human “CTLH” multiprotein E3 ligases to tightly bind a wide range of N-terminal residues whose recognition is determined in part by the downstream sequence context. Screening of phage displaying peptide libraries with exposed N-termini identified novel consensus motifs with non-Pro N-terminal residues binding Gid4 or Gid10 with high affinity. Structural data reveal that conformations of flexible loops in Gid4 and Gid10 complement sequences and folds of interacting peptides. Together with analysis of endogenous substrate degrons, the data show that degron identity, substrate domains harboring targeted lysines, and varying E3 ligase higher-order assemblies combinatorially determine efficiency of ubiquitylation and degradation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0022-2836 1089-8638 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34767800; info:eu-repo/semantics/altIdentifier/wos/WOS:000725064200007; info:eu-repo/semantics/altIdentifier/isbn/0022-2836; info:eu-repo/semantics/; https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=63611; urn:isbn:0022-2836; urn:issn:0022-2836; urn:issn:1089-8638 |
| DOI: | 10.1016/j.jmb.2021.167347 |
| الاتاحة: | https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=63611 https://doi.org/10.1016/j.jmb.2021.167347 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.CC91118 |
| قاعدة البيانات: | BASE |
| تدمد: | 00222836 10898638 |
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| DOI: | 10.1016/j.jmb.2021.167347 |