Academic Journal
Decoding Molecular Mechanism Underlying Human Olfactory Receptor OR8D1 Activation by Sotolone Enantiomers
| العنوان: | Decoding Molecular Mechanism Underlying Human Olfactory Receptor OR8D1 Activation by Sotolone Enantiomers |
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| المؤلفون: | Juan Wang, Danqing Wang, Mingquan Huang, Baoguo Sun, Fazheng Ren, Jihong Wu, Jinglin Zhang, Hehe Li, Xiaotao Sun |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biochemistry, Cell Biology, Genetics, Molecular Biology, Pharmacology, Biotechnology, Developmental Biology, Cancer, Computational Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, multiple sequence alignments, molecular dynamics simulations, based luminescence assay, 98 ± 1, recombinant odorant receptors, sotolone enantiomers sotolone, 20 ± 0, 50 , 46 , >)- sotolone, odorant docking, r <, respectively ), potent agonist, odor characteristics, nutty aroma, important role, green aroma |
| الوصف: | Sotolone, a chiral compound, plays an important role in the food industry. Herein, ( R )-/( S )-sotolone were separated to determine their odor characteristics and thresholds in air ( R -form: smoky, burned, herb, and green aroma, 0.0514 μg/m 3 ; S -form: sweet, milk, acid, and nutty aroma, 0.0048 μg/m 3 ). OR8D1 responses to ( R )-/( S )-sotolone were detected in a HEK293 cell-based luminescence assay. ( S )-Sotolone was a more potent agonist than ( R )-sotolone (EC 50 values of 84.98 ± 1.05 and 167.20 ± 0.25 μmol/L, respectively). Molecular dynamics simulations and molecular mechanics Poisson–Boltzmann surface area analyses confirmed that the combination of ( S )-sotolone and OR8D1 was more stable than that of ( R )-sotolone. Odorant docking, multiple sequence alignments, site-directed mutagenesis, and functional studies with recombinant odorant receptors (ORs) in a cell-based luminescence assay identified 11 amino-acid residues that influence the enantioselectivity of OR8D1 toward sotolone significantly and that N206 5.46 was indispensable to the activation of OR8D1 by ( S )-sotolone. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Decoding_Molecular_Mechanism_Underlying_Human_Olfactory_Receptor_OR8D1_Activation_by_Sotolone_Enantiomers/25269976 |
| DOI: | 10.1021/acs.jafc.3c09142.s001 |
| الاتاحة: | https://doi.org/10.1021/acs.jafc.3c09142.s001 https://figshare.com/articles/journal_contribution/Decoding_Molecular_Mechanism_Underlying_Human_Olfactory_Receptor_OR8D1_Activation_by_Sotolone_Enantiomers/25269976 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.CC8E9D1C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jafc.3c09142.s001 |
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