Academic Journal
Identification of Candidate Genes Induced by Retinoic Acid in Embryonal Carcinoma Cells
| العنوان: | Identification of Candidate Genes Induced by Retinoic Acid in Embryonal Carcinoma Cells |
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| المؤلفون: | Cheung, William M. W., Chu, Apple H., Ip, Nancy Y. |
| المصدر: | Journal of Neurochemistry ; volume 68, issue 5, page 1882-1888 ; ISSN 0022-3042 1471-4159 |
| بيانات النشر: | Wiley |
| سنة النشر: | 1997 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Retinoic acid (RA) induced the terminal differentiation of a human embryonal carcinoma cell line (NT2/D1) into several morphologically distinct cell types, including the postmitotic CNS neurons. Although RA has been suggested to play an important role in brain development, little is known about the molecular mechanism by which RA induces neuronal differentiation. In the present study, RNA fingerprinting by arbitrarily primed PCR (RAP‐PCR) was used to identify the transcripts in NT2/D1 cells that were differentially regulated by RA. Northern blot analysis of the differentially amplified PCR fragments revealed 11 genes that were regulated by RA. Of these, seven were up‐regulated and four were down‐regulated along the course of RA treatment. More importantly, four of the RA‐regulated genes that were identified in the present study are novel. Our findings suggested that there are a number of RA‐regulated genes that have yet to be identified. RAP‐PCR provides a useful tool for studying the patterns of transcript expression during the course of RA treatment and allows the cloning of novel genes involved in the process of neuronal differentiation. Furthermore, it provides a basis for the selection of genes that are involved in the RA‐induced signaling pathway in the human CNS. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1046/j.1471-4159.1997.68051882.x |
| الاتاحة: | http://dx.doi.org/10.1046/j.1471-4159.1997.68051882.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-4159.1997.68051882.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-4159.1997.68051882.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.CC8CBB11 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1046/j.1471-4159.1997.68051882.x |
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