Academic Journal
Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2
| العنوان: | Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2 |
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| المؤلفون: | Kim, Juhyun, Goñi-Moreno, Ángel, de Lorenzo, Victor |
| المساهمون: | Ministerio de Ciencia e Innovación (España), European Commission, Comunidad de Madrid, Agencia Estatal de Investigación (España), Goñi-Moreno, Angel, de Lorenzo, Victor |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2021 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Pseudomonas putida, TOL plasmid, Biodegradation, m-xylene, mRNA, mRNA localization, Nucleoid, Specific DNA locus, Stress adaptation, Transcriptional regulation, Translational control, xylUW, xylX |
| الوصف: | Centro de Biotecnología y Genómica de Plantas (CBGP) ; Despite intensive research on the biochemical and regulatory features of the archetypal catabolic TOL system borne by pWW0 of Pseudomonas putida strain mt-2, the physical arrangement and tridimensional logic of the xyl gene expression flow remains unknown. In this work, the spatial distribution of specific xyl mRNAs with respect to the host nucleoid, the TOL plasmid, and the ribosomal pool has been investigated. In situ hybridization of target transcripts with fluorescent oligonucleotide probes revealed that xyl mRNAs cluster in discrete foci, adjacent but clearly separated from the TOL plasmid and the cell nucleoid. Also, they colocalize with ribosome-rich domains of the intracellular milieu. This arrangement was maintained even when the xyl genes were artificially relocated to different chromosomal locations. The same held true when genes were expressed through a heterologous T7 polymerase-based system, which likewise led to mRNA foci outside the DNA. In contrast, rifampin treatment, known to ease crowding, blurred the confinement of xyl transcripts. This suggested that xyl mRNAs exit from their initiation sites to move to ribosome-rich points for translation-rather than being translated coupled to transcription. Moreover, the results suggest the distinct subcellular motion of xyl mRNAs results from both innate properties of the sequences and the physical forces that keep the ribosomal pool away from the nucleoid in P. putida This scenario is discussed within the background of current knowledge on the three-dimensional organization of the gene expression flow in other bacteria and the environmental lifestyle of this soil microorganism.IMPORTANCE The transfer of information between DNA, RNA, and proteins in a bacterium is often compared to the decoding of a piece of software in a computer. However, the tridimensional layout and the relational logic of the cognate biological hardware, i.e., the nucleoid, the RNA polymerase, and the ribosomes, are habitually ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2161-2129 |
| Relation: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095584-B-C42/ES/PROGRAMACION GENETICA DE PSEUDOMONAS PUTIDA PARA UNA EFICACIA CATALITICA SOSTENIDA A TRAVES DE LA ESCALA DE DISPONIBILIDAD DE AGUA/; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCI2019-111859-2/ES/COMUNIDADES MICROBIANAS SINTETICAS PARA LA PRODUCCION DE PRODUCTOS DERIVADOS DEL LIMONENO/; info:eu-repo/grantAgreement/EC/H2020/766975; info:eu-repo/grantAgreement/EC/H2020/820699; info:eu-repo/grantAgreement/EC/H2020/814650; info:eu-repo/grantAgreement/EC/H2020/870294; 2019-T1/BIO-14053/Atracción deTalento Program; info:eu-repo/grantAgreement/AEI//SEV-2016-0672 (2017-2021).; mBio; Publisher's version; https://doi.org/10.1128/mBio.03685-20; Sí; mBio 12(1): e03685 (2021); http://hdl.handle.net/10261/271887; http://dx.doi.org/10.13039/501100004837; http://dx.doi.org/10.13039/501100011033; http://dx.doi.org/10.13039/100012818; http://dx.doi.org/10.13039/501100000780; 2-s2.0-85101389860; https://api.elsevier.com/content/abstract/scopus_id/85101389860 |
| DOI: | 10.1128/mBio.03685-20 |
| DOI: | 10.13039/501100004837 |
| DOI: | 10.13039/501100011033 |
| DOI: | 10.13039/100012818 |
| DOI: | 10.13039/501100000780 |
| الاتاحة: | http://hdl.handle.net/10261/271887 https://doi.org/10.1128/mBio.03685-20 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/100012818 https://doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85101389860 |
| Rights: | open |
| رقم الانضمام: | edsbas.CC82E8B3 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21612129 |
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| DOI: | 10.1128/mBio.03685-20 |