التفاصيل البيبلوغرافية
| العنوان: |
Shotgun Metaproteomics of the Human Distal Gut Microbiota |
| المؤلفون: |
VerBerkmoes, N. C., Russell, A. L., Shah, M., Godzik, A., Rosenquist, M., Halfvarsson, J., Lefsrud, M. G., Apajalahti, J., Tysk, C., Hettich, R. L., Jansson, Janet K. |
| المساهمون: |
Lawrence Berkeley National Laboratory. Earth Sciences Division. |
| المصدر: |
Journal Name: International Society for Microbial Ecology Journal |
| بيانات النشر: |
Lawrence Berkeley National Laboratory |
| سنة النشر: |
2008 |
| المجموعة: |
University of North Texas: UNT Digital Library |
| مصطلحات موضوعية: |
Genes, Carbohydrates, Proteins Microbe, Microbe Host Interactions, Microbiome, Metagenomics, Production, Peptides, Distribution, Metabolism, Microbe |
| Time: |
58 |
| الوصف: |
The human gut contains a dense, complex and diverse microbial community, comprising the gut microbiome. Metagenomics has recently revealed the composition of genes in the gut microbiome, but provides no direct information about which genes are expressed or functioning. Therefore, our goal was to develop a novel approach to directly identify microbial proteins in fecal samples to gain information about the genes expressed and about key microbial functions in the human gut. We used a non-targeted, shotgun mass spectrometry-based whole community proteomics, or metaproteomics, approach for the first deep proteome measurements of thousands of proteins in human fecal samples, thus demonstrating this approach on the most complex sample type to date. The resulting metaproteomes had a skewed distribution relative to the metagenome, with more proteins for translation, energy production and carbohydrate metabolism when compared to what was earlier predicted from metagenomics. Human proteins, including antimicrobial peptides, were also identified, providing a non-targeted glimpse of the host response to the microbiota. Several unknown proteins represented previously undescribed microbial pathways or host immune responses, revealing a novel complex interplay between the human host and its associated microbes. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
52 p.; ill.; Text |
| اللغة: |
English |
| Relation: |
rep-no: LBNL-1134E; grantno: DE-AC02-05CH11231; osti: 943452; https://digital.library.unt.edu/ark:/67531/metadc894170/; ark: ark:/67531/metadc894170 |
| الاتاحة: |
https://digital.library.unt.edu/ark:/67531/metadc894170/ |
| رقم الانضمام: |
edsbas.CC6257EB |
| قاعدة البيانات: |
BASE |