Image
Binding of soluble CD4 to ⍺ 4 β 7 on CD4 + T cells.
| العنوان: | Binding of soluble CD4 to ⍺ 4 β 7 on CD4 + T cells. |
|---|---|
| المؤلفون: | Donald Van Ryk, Sinmanus Vimonpatranon, Joe Hiatt, Sundar Ganesan, Nathalie Chen, Jordan McMurry, Saadiq Garba, Susie Min, Livia R. Goes, Alexandre Girard, Jason Yolitz, Isabella Licavoli, Danlan Wei, Dawei Huang, Marcelo A. Soares, Elena Martinelli, Claudia Cicala, James Arthos |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Neuroscience, Biotechnology, Evolutionary Biology, Developmental Biology, Cancer, Plasma Physics, Mental Health, Infectious Diseases, Plant Biology, Virology, Computational Biology, terminal igsf domains, div >< p, two recognized ligands, 43 , cd4 tropic virus, ⍺< sub xmlns, sup >+, mhc ii interactions, cis regulatory interaction, 1 &# 176, hiv docking receptor, hiv gp120 mimics, cd4 binds directly |
| الوصف: | (A) Schematic of D1D2-Ig⍺tp. (B) Flow-cytometry histogram of PE-conjugated D1D2-Ig⍺tp staining of CD4 + T cells alone (blue) or in the presence of either the anti ⍺ 4 mAb 2B4 (green) or an irrelevant mAb (black-dashed). Cells stained with PE-conjugated MicA-Ig⍺tp (red), along with unstained cells (grey) are included. X-axis indicates geometric mean fluorescence intensity (gMFI). (C) 293T cells untransfected or co-transfected with ⍺ 4 and β 7 expression vectors stained with either PE-D1D2-Ig⍺tp or PE-MAdCAM-Ig in the absence or presence of the ⍺ 4 antagonist FG. Y-axis indicates gMFI. (D) CD4 + T cells stained with anti CD45RO (x-axis) anti β 7 (y-axis) (left), or with anti CD45RO (x-axis) and D1D2-Ig⍺tp (y-axis) (right). % cells in each quadrant, as indicated. (E) CD4 + T cells stained as in B, with lower MW forms of oligomerized D1D2-Ig⍺tp. Left Y-axis indicates gMFI (bars), right Y-axis indicates relative MW (kDa) (•). (F) Staining of primary CD4 + T cells with PE-conjugated D1D2-Ig⍺tp, PE-MAdCAM-Ig or PE-VCAM-Ig, as in panel B in the presence of buffers containing different combinations of CaCl 2 , MgCl 2 and MnCl 2 . Y-axis indicates gMFI. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Binding_of_soluble_CD4_to_sub_4_sub_sub_7_sub_on_CD4_sup_sup_T_cells_/24776396 |
| DOI: | 10.1371/journal.ppat.1011860.g001 |
| الاتاحة: | https://doi.org/10.1371/journal.ppat.1011860.g001 https://figshare.com/articles/figure/Binding_of_soluble_CD4_to_sub_4_sub_sub_7_sub_on_CD4_sup_sup_T_cells_/24776396 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.CC2396A5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.ppat.1011860.g001 |
|---|