Academic Journal
Scn1a-GFP transgenic mouse revealed Nav1.1 expression in neocortical pyramidal tract projection neurons
| العنوان: | Scn1a-GFP transgenic mouse revealed Nav1.1 expression in neocortical pyramidal tract projection neurons |
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| المؤلفون: | Yamagata, Tetsushi, Ogiwara, Ikuo, Tatsukawa, Tetsuya, Suzuki, Toshimitsu, Otsuka, Yuka, Imaeda, Nao, Mazaki, Emi, Inoue, Ikuyo, Tokonami, Natsuko, Hibi, Yurina, Itohara, Shigeyoshi, Yamakawa, Kazuhiro |
| المساهمون: | Japan Society for the Promotion of Science, RIKEN Center for Brain Science, Japan Agency for Medical Research and Development, Ministry of Education, Culture, Sports, Science and Technology, Takeda Science Foundation, Kiyokun Foundation, Japan Epilepsy Research Foundation |
| المصدر: | eLife ; volume 12 ; ISSN 2050-084X |
| بيانات النشر: | eLife Sciences Publications, Ltd |
| سنة النشر: | 2023 |
| المجموعة: | eLife (E-Journal - via CrossRef) |
| الوصف: | Expressions of voltage-gated sodium channels Nav1.1 and Nav1.2, encoded by SCN1A and SCN2A genes, respectively, have been reported to be mutually exclusive in most brain regions. In juvenile and adult neocortex, Nav1.1 is predominantly expressed in inhibitory neurons while Nav1.2 is in excitatory neurons. Although a distinct subpopulation of layer V (L5) neocortical excitatory neurons were also reported to express Nav1.1, their nature has been uncharacterized. In hippocampus, Nav1.1 has been proposed to be expressed only in inhibitory neurons. By using newly generated transgenic mouse lines expressing Scn1a promoter-driven green fluorescent protein (GFP), here we confirm the mutually exclusive expressions of Nav1.1 and Nav1.2 and the absence of Nav1.1 in hippocampal excitatory neurons. We also show that Nav1.1 is expressed in inhibitory and a subpopulation of excitatory neurons not only in L5 but all layers of neocortex. By using neocortical excitatory projection neuron markers including FEZF2 for L5 pyramidal tract (PT) and TBR1 for layer VI (L6) cortico-thalamic (CT) projection neurons, we further show that most L5 PT neurons and a minor subpopulation of layer II/III (L2/3) cortico-cortical (CC) neurons express Nav1.1 while the majority of L6 CT, L5/6 cortico-striatal (CS), and L2/3 CC neurons express Nav1.2. These observations now contribute to the elucidation of pathological neural circuits for diseases such as epilepsies and neurodevelopmental disorders caused by SCN1A and SCN2A mutations. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7554/elife.87495 |
| الاتاحة: | http://dx.doi.org/10.7554/elife.87495 https://cdn.elifesciences.org/articles/87495/elife-87495-v1.pdf https://cdn.elifesciences.org/articles/87495/elife-87495-v1.xml https://elifesciences.org/articles/87495 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.CBA89DCC |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/elife.87495 |
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