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Image_1_Comparison of gut microbiota immunity and pathology in specific-pathogen-free chickens with glandular and muscular gastritis using different methods.jpg
| العنوان: | Image_1_Comparison of gut microbiota immunity and pathology in specific-pathogen-free chickens with glandular and muscular gastritis using different methods.jpg |
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| المؤلفون: | Zunxiang Yan, Shifa Yang, Shuqian Lin, Zengcheng Zhao, Yueyue Liu, Bin Yin, Yunpeng Yi, Shikai Song, Rongling Zhang, Zhongli Huang |
| سنة النشر: | 2024 |
| المجموعة: | Frontiers: Figshare |
| مصطلحات موضوعية: | Animal Systematics and Taxonomy, Animal Physiology - Biophysics, Animal Physiology - Cell, Animal Physiology - Systems, Animal Behaviour, Animal Cell and Molecular Biology, Animal Developmental and Reproductive Biology, Animal Immunology, Animal Neurobiology, Animal Physiological Ecology, Animal Structure and Function, Veterinary Anaesthesiology and Intensive Care, Veterinary Anatomy and Physiology, Veterinary Diagnosis and Diagnostics, Veterinary Epidemiology, Veterinary Immunology, Veterinary Medicine, Veterinary Microbiology (excl. Virology), Veterinary Parasitology, Veterinary Pathology, Veterinary Pharmacology, Veterinary Surgery, Veterinary Virology, Veterinary Sciences not elsewhere classified, glandular and muscular gastritis, SPF chicken, animal model, 16S rDNA, gut microbiome |
| الوصف: | The objective of this study is to review different methods to screen for the optimal model for preventing and treating chicken glandular and muscular gastritis syndrome. Twenty-four 40-day-old specific pathogen-free (SPF) chickens were randomly allocated into four groups (N = 6): polyethylene glycol + ammonium chloride group (M1 group), acetic acid + rhubarb group (M2 group), polyethylene glycol + rhubarb group (M3 group), and control group. The control group had free access to water, while the remaining groups received different doses of molding reagents added to their drinking water. The animal models were assessed based on clinical manifestations, histopathology findings, serological analysis, and composition of intestinal microbiota to establish an optimal approach for constructing an avian model of glandular and muscular gastritis. The SPF chickens in each model group exhibited typical symptoms of glandular and muscular gastritis, poor spirit, yellow loose stools with undigested feed, and enlargement and ulceration of the glandular and muscular stomach. Among these groups, the M3 group had the highest incidence rate of 100%. Compared to the control group, the body weight and body temperature of the chicken in the three model groups were reduced, and the glandular and muscular stomachs and duodenum showed different degrees of bleeding, mucosal abscission, and other pathological injuries. Additionally, the levels of serum IL-2 and α-amylase activity decreased while the content of IL-4 increased. After conducting 16s rDNA sequencing, it was observed that the abundance of Bacteroides, Faecalibacterium, and Ruminococcaceae UCG-014 was significantly increased in the model group compared to the control group. Conversely, there was a notable decrease in the levels of Megamonas and Lactobacillus, which are speculated to be associated with arachidonic acid metabolism, the NF-κB signaling pathway, and TNF signaling pathways. The combination of polyethylene glycol and rhubarb emerged as the most effective method for ... |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Image_1_Comparison_of_gut_microbiota_immunity_and_pathology_in_specific-pathogen-free_chickens_with_glandular_and_muscular_gastritis_using_different_methods_jpg/25955647 |
| DOI: | 10.3389/fvets.2024.1343768.s001 |
| الاتاحة: | https://doi.org/10.3389/fvets.2024.1343768.s001 https://figshare.com/articles/figure/Image_1_Comparison_of_gut_microbiota_immunity_and_pathology_in_specific-pathogen-free_chickens_with_glandular_and_muscular_gastritis_using_different_methods_jpg/25955647 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.CB9374F |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fvets.2024.1343768.s001 |
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