Academic Journal
Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure.
| العنوان: | Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure. |
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| المؤلفون: | Rodrigues, Olivier, Reshetnyak, Ganna, Grondin, Alexandre, Saijo, Yusuke, Leonhardt, Nathalie, Maurel, Christophe, Verdoucq, Lionel |
| المساهمون: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Department of Biology, Northern Arizona University Flagstaff, Biologie végétale et microbiologie environnementale - UMR7265 (BVME), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Plant Environmental Physiology and Stress Signaling (PEPSS), European Project: Erasmus |
| المصدر: | ISSN: 0027-8424. |
| بيانات النشر: | HAL CCSD National Academy of Sciences |
| سنة النشر: | 2017 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | aquaporin, guard cell signaling, hydrogen peroxide, pathogen, stomatal movement, stomata, guard cell, cellule de garde, membrane plasmique périacrosomique, arabidopsis, mouvement stomatique, stomate, peroxyde d'hydrogène, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
| الوصف: | Stomatal movements are crucial for the control of plant water status and protection against pathogens. Assays on epidermal peels revealed that, similar to abscisic acid (ABA), pathogen-associated molecular pattern (PAMP) flg22 requires the AtPIP2;1 aquaporin to induce stomatal closure. Flg22 also induced an increase in osmotic water permeability (Pf) of guard cell protoplasts through activation of AtPIP2;1. The use of HyPer, a genetic probe for intracellular hydrogen peroxide (H2O2), revealed that both ABA and flg22 triggered an accumulation of H2O2 in wild-type but not pip2;1 guard cells. Pretreatment of guard cells with flg22 or ABA facilitated the influx of exogenous H2O2 Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) and open stomata 1 (OST1)/Snf1-related protein kinase 2.6 (SnRK2.6) were both necessary to flg22-induced Pf and both phosphorylated AtPIP2;1 on Ser121 in vitro. Accumulation of H2O2 and stomatal closure as induced by flg22 was restored in pip2;1 guard cells by a phosphomimetic form (Ser121Asp) but not by a phosphodeficient form (Ser121Ala) of AtPIP2;1. We propose a mechanism whereby phosphorylation of AtPIP2;1 Ser121 by BAK1 and/or OST1 is triggered in response to flg22 to activate its water and H2O2 transport activities. This work establishes a signaling role of plasma membrane aquaporins in guard cells and potentially in other cellular context involving H2O2 signaling. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28784763; hal-01595145; https://hal.science/hal-01595145; https://hal.science/hal-01595145/document; https://hal.science/hal-01595145/file/RodriguesO.-et%20al-PNAS-2017_1.pdf; PRODINRA: 405144; PUBMED: 28784763; WOS: 000408095300077 |
| DOI: | 10.1073/pnas.1704754114 |
| الاتاحة: | https://hal.science/hal-01595145 https://hal.science/hal-01595145/document https://hal.science/hal-01595145/file/RodriguesO.-et%20al-PNAS-2017_1.pdf https://doi.org/10.1073/pnas.1704754114 |
| Rights: | http://creativecommons.org/licenses/by-sa/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.CB2DEEFC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1073/pnas.1704754114 |
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