Academic Journal
Basic cell penetrating peptides induce plasma membrane positive curvature, lipid domain separation and protein redistribution
| العنوان: | Basic cell penetrating peptides induce plasma membrane positive curvature, lipid domain separation and protein redistribution |
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| المؤلفون: | Maniti, Ofelia, Piao, Hong-Rong, Ayala-Sanmartin, Jesus |
| المساهمون: | Génie Enzymatique, Membrane Biomimétique et Assemblages Supramoléculaires (GEMBAS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Trafic Membranaire et Signalisation Dans les Cellules Epitheliales, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | EISSN: 1687-8884 ; International Journal of Biochemistry and Cell Biology ; https://hal.science/hal-02345708 ; International Journal of Biochemistry and Cell Biology, 2014 |
| بيانات النشر: | HAL CCSD Hindawi Pub. Corp. |
| سنة النشر: | 2014 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | Basic peptides, membrane curvature, membrane domains, membrane rolling, plasma membrane 2 Abbreviations: CPP, Cell Penetratin Peptide, Plasma Membrane Sphere, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] |
| الوصف: | International audience ; Basic cell penetrating peptides are tools for molecular cellular internalization of non membrane permeable molecules. Their uptake mechanisms involve energy-dependent and energy-independent pathways such as endocytosis, direct translocation or physical endocytosis. These mechanisms are ruled by both, the peptides physicochemical properties and structure and by the membrane lipids characteristics and organisation. Herein we used plasma membrane spheres and membrane models to study the membrane perturbations induced by three arginine-rich cell penetrating peptides. Nona-arginine (R9) and the amphipathic peptide RWRRWWRRW (RW9) induced positive membrane curvature in the form of buds and membrane tubes. Membranous tubes underwent rolling resulting in formation of multilamellar membrane particles at the surface of the plasma membrane spheres. The amphipathic peptides RW9 and RRWRRWWRRWWRRWRR (RW16) provoked lipid and membrane associated protein domain separation as well as changes in membrane fluidity and cholesterol redistribution. These data suggest that membrane domains separation and the formation of multilamellar membranous particles would be involved in arginine-rich cell penetrating peptides internalization. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-02345708; https://hal.science/hal-02345708; https://hal.science/hal-02345708/document; https://hal.science/hal-02345708/file/Main%20text%20Ayala-Sanmartin.pdf |
| الاتاحة: | https://hal.science/hal-02345708 https://hal.science/hal-02345708/document https://hal.science/hal-02345708/file/Main%20text%20Ayala-Sanmartin.pdf |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.CB028FF2 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |