Academic Journal
Dipstick Test for Rapid Diagnosis of Shigella dysenteriae 1 in Bacterial Cultures and Its Potential Use on Stool
| العنوان: | Dipstick Test for Rapid Diagnosis of Shigella dysenteriae 1 in Bacterial Cultures and Its Potential Use on Stool |
|---|---|
| المؤلفون: | Neelam Taneja, Faridabano Nato, Sylvie Dartevelle, Jean Marie Sire, Benoit Garin, Lan Nguyen Thi, Tai The Diep, Jean Christophe Shako, Ingrid Filliol, Marie Noëlle Ungeheuer, Catherine Ottone, Yves Germani |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://hal-riip.archives-ouvertes.fr/docs/00/83/65/03/PDF/21984895TanejaPLoSOne2011_6_10_e24830.pdf. |
| المجموعة: | CiteSeerX |
| الوصف: | Background: We describe a test for rapid detection of S. dysenteriae 1 in bacterial cultures and in stools, at the bedside of patients. Methodology/Principal Findings: The test is based on the detection of S. dysenteriae 1 lipopolysaccharide (LPS) using serotype 1-specific monoclonal antibodies coupled to gold particles and displayed on a one-step immunochromatographic dipstick. A concentration as low as 15 ng/ml of LPS was detected in distilled water and in reconstituted stools in 10 minutes. In distilled water and in reconstituted stools, an unequivocal positive reaction was obtained with 1.66106 CFU/ml and 4.96106 CFU/ml of S. dysenteriae 1, respectively. Optimal conditions to read the test have been determined to limit the risk of ambiguous results due to appearance of a faint yellow test band in some negative samples. The specificity was 100% |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.635.6657; http://hal-riip.archives-ouvertes.fr/docs/00/83/65/03/PDF/21984895TanejaPLoSOne2011_6_10_e24830.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.635.6657 http://hal-riip.archives-ouvertes.fr/docs/00/83/65/03/PDF/21984895TanejaPLoSOne2011_6_10_e24830.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.CAFB28C7 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |