Conference
Influence of bone substitute PerOssal® on bone marrrow mesenchymal stem cells
| العنوان: | Influence of bone substitute PerOssal® on bone marrrow mesenchymal stem cells |
|---|---|
| المؤلفون: | Obradović, Hristina, Häusner, Sebastian, Trivanović, Drenka, Živanović, Milena, Spasovski, Duško, Herrmann, Marietta, Jauković, Aleksandra |
| المصدر: | Hemijska industrija |
| بيانات النشر: | Savez hemijskih inženjera Srbije |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Mesenchymal stem cells, bone regeneration, cell migration, osteogenesis |
| الوصف: | INTRODUCTION: PerOssal® is biologically degradable and osteoconductive bone substitute consisting of nanocrystalline hydroxyapatite (51.5 %) and calcium sulfate (48.5 %) [1]. It is used for bone defect treatments as a synthetic carrier for antibiotics suitable for infected areas. Since the effect of PerOssal® has not been investigated so far in combination with mesenchymal stem cells (MSC) for bone tissue regeneration, we aimed to explore in vitro whether this material can support growth and osteogenesis of MSC from bone marrow (BM-MSC). Materials and Methods: BM-MSC were isolated from BM mononuclear cell fraction of human healthy donors and cultivated in standard conditions. One pellet of PerOssal® was dissolved in 10 ml of Phosphate Buffered Saline and added to cells in different ratios. Viability of BM-MSC was assessed by MTT test [2] after one, five and seven days. Migration capacity of BM-MSC was followed by both scratch assay and transwell system [3], while osteogenic potential was investigated by histochemical staining after induced osteogenic differentiation [4]. RESULTS AND DISCUSSION: Initial experiments showed that BM-MSC adhere to PerOssal® surface. Considering that culture media induced decomposition of PerOssal® which aggravated functional evaluation of attached BM-MSC , our further in vitro studies were directed on testing the effects of different PerOssal® dilutions on BM-MSC functional properties. Our results demonstrated that at all dilutions tested PerOssal® didn’t affect viability of BM-MSC in a shortterm treatment, while in higher doses it decreased cell viability at day 7. At the same time point, morphology of BMMSC was changed with high dose of PerOssal®. On the other side, at lower doses PerOssal® stimulated migration of BMMSC as demonstrated by both scratch and transwell assays. As for the analyses of BM-MSC osteogenic differentiation, stimulatory effect on early osteogenesis was noticed for lower PerOssal® doses, opposite to decreased BM-MSC osteogenesis observed when higher doses were ... |
| نوع الوثيقة: | conference object |
| اللغة: | English |
| Relation: | Research and development project between Germany and the Western Balkan states - RIABONE (01DS21004); 0367–598X; https://rimi.imi.bg.ac.rs/handle/123456789/1560; http://rimi.imi.bg.ac.rs/bitstream/id/3729/Influence_of_bone_substitute_PerOssal_HEMIND.pdf; https://hdl.handle.net/21.15107/rcub_rimi_1560 |
| الاتاحة: | https://rimi.imi.bg.ac.rs/handle/123456789/1560 http://rimi.imi.bg.ac.rs/bitstream/id/3729/Influence_of_bone_substitute_PerOssal_HEMIND.pdf https://hdl.handle.net/21.15107/rcub_rimi_1560 |
| Rights: | openAccess ; https://creativecommons.org/licenses/by/4.0/ ; BY |
| رقم الانضمام: | edsbas.CAEE0C29 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |