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Image_2_Antibody-Dependent Cellular Phagocytosis of HIV-1-Infected Cells Is Efficiently Triggered by IgA Targeting HIV-1 Envelope Subunit gp41.TIFF
| العنوان: | Image_2_Antibody-Dependent Cellular Phagocytosis of HIV-1-Infected Cells Is Efficiently Triggered by IgA Targeting HIV-1 Envelope Subunit gp41.TIFF |
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| المؤلفون: | Maxence Duchemin, Daniela Tudor, Andréa Cottignies-Calamarte, Morgane Bomsel |
| سنة النشر: | 2020 |
| المجموعة: | Frontiers: Figshare |
| مصطلحات موضوعية: | Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, IgA, HIV-1 envelope gp41, phagocytosis, ADCP, FcαRI/CD89, neutrophils, monocytes |
| الوصف: | Antibodies mediate a broad array of non-neutralizing Fc-mediated functions against HIV-1 including antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP). Accordingly, ADCC and ADCP induced by anti-HIV envelope gp120 IgG have been correlated to the limited success of the HIV-1 phase III vaccine trial RV144. It remains elusive whether ADCP can also be triggered by IgA, the isotype predominant at mucosal surfaces through which HIV-1 is mainly transmitted. Yet, we have previously shown that the HIV envelope subunit gp41-specific broadly neutralizing antibody 2F5 under the IgA isotype (2F5-IgA) triggers ADCC and cooperates with 2F5-IgG to increase HIV-1-infected cell lysis. Here, we now demonstrate that 2F5-IgA, more efficiently than 2F5-IgG, induces ADCP not only of gp41-coated beads but also of primary HIV-1-infected cells in a FcαRI-dependent manner. Both primary monocytes and neutrophils can act as effector cells of 2F5-IgA-mediated ADCP, although with different kinetics with faster neutrophil phagocytosis. However, unlike for ADCC, 2F5-IgA and 2F5-IgG do not cooperate to increase ADCP. Altogether, our results reveal that gp41-specific IgA mediate the efficient phagocytosis of HIV-1-infected cells. Inducing such ADCC and ADCP-prone IgA response by vaccination in addition to anti-HIV envelope IgG, might increase the protection against HIV acquisition at mucosal level. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Image_2_Antibody-Dependent_Cellular_Phagocytosis_of_HIV-1-Infected_Cells_Is_Efficiently_Triggered_by_IgA_Targeting_HIV-1_Envelope_Subunit_gp41_TIFF/12451178 |
| DOI: | 10.3389/fimmu.2020.01141.s002 |
| الاتاحة: | https://doi.org/10.3389/fimmu.2020.01141.s002 https://figshare.com/articles/figure/Image_2_Antibody-Dependent_Cellular_Phagocytosis_of_HIV-1-Infected_Cells_Is_Efficiently_Triggered_by_IgA_Targeting_HIV-1_Envelope_Subunit_gp41_TIFF/12451178 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.CAB0D27C |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2020.01141.s002 |
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