Academic Journal
Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water ; Avaliação da técnica de detecção por fluorescência como alternativa para contagem de bactérias heterotróficas em água para hemodiálise
| العنوان: | Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water ; Avaliação da técnica de detecção por fluorescência como alternativa para contagem de bactérias heterotróficas em água para hemodiálise |
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| المؤلفون: | Hilinski, Ellen Gameiro, Bugno, Adriana, Silva, Fernando Pontes de Lima e, Almodovar, Adriana Aparecida Buzzo, Pinto, Terezinha de Jesus Andreoli |
| المصدر: | Health Surveillance under Debate: Society, Science & Technology ; Vol. 8 No. 2 (2020): May; 94-105 ; Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 8 Núm. 2 (2020): Puede; 94-105 ; Vigil Sanit Debate, Rio de Janeiro; v. 8 n. 2 (2020): Maio; 94-105 ; 2317-269X |
| بيانات النشر: | Instituto Nacional de Controle de Qualidade em Saúde |
| سنة النشر: | 2020 |
| المجموعة: | Revista Visa em Debate (Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia) |
| مصطلحات موضوعية: | Hemodialysis, Treated Water, Heterotrophic Bacteria, Fluorescence, Hemodiálise, Água Tratada, Bactérias Heterotróficas, Fluorescência |
| الوصف: | Introduction: The microbiological quality of hemodialysis treated water is directly related to the occurrence of infections and pyrogenic reactions in patients. Objective: Determine the minimum incubation time and evaluate the alternative microbiological method performance for heterotrophic bacteria count in hemodialysis water through the fluorescence microbial detection technique. Method: The analyses were conducted by concentration levels of 2,5 x 10-1 to 1,0 x 102 CFU/plate for Pseudomonas aeruginosa, Burkholderia cepacia, Escherichia coli and Staphylococcus aureus. The tests were performed simultaneously by the alternative and the traditional methods, using culture medium R2A and incubation temperature of 24.0°C ± 4.0°C. The incubation times were 40 h and 120 h, respectively. Fourteen hemodialysis water samples were analyzed to assess the equivalence between the methods evaluated. Results: The results demonstrated that the alternative method allows quantification of heterotrophic bacteria after 40 h of incubation, with accuracy, precision, specificity and linearity for the range of 5 to 100 CFU/plate. The detection limit of the alternative method is 1 CFU/plate. Conclusions: It was possible to conclude that the alternative method has equivalent results to the traditional method, since the confidence interval of the alternative method was entirely within the equivalence range. Therefore, the microbial detection technique by fluorescence showed a viable option for the implementation of a rapid microbiological method for the heterotrophic bacteria count in samples of treated water for hemodialysis. ; Introdução: A qualidade microbiológica da água tratada para hemodiálise está diretamente relacionada à ocorrência de infecções e de reações pirogênicas nos pacientes. Objetivo: Determinar o tempo de incubação mínimo e avaliar o desempenho do método microbiológico alternativo para a contagem de bactérias heterotróficas em água de hemodiálise por meio da técnica de detecção microbiana por fluorescência. Método: As ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Portuguese English |
| Relation: | https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387/1220; https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387/1221; https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387 |
| الاتاحة: | https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387 |
| Rights: | Copyright (c) 2020 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debate ; https://creativecommons.org/licenses/by/3.0/deed.pt_BR |
| رقم الانضمام: | edsbas.CAB04A8F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |