Academic Journal
Effect of testicular tissue lysate on developmental competence of porcine oocytes matured and fertilized in vitro
| العنوان: | Effect of testicular tissue lysate on developmental competence of porcine oocytes matured and fertilized in vitro |
|---|---|
| المؤلفون: | Singh, AK, Naskar, S, Saikia, B, Vashi, Y, Gupta, S, Banik, S, Tamuli, MK, Pande, V, Sarma, DK, Dhara, SK |
| المساهمون: | Indian Council of Agricultural Research |
| المصدر: | Reproduction in Domestic Animals ; volume 52, issue 2, page 183-188 ; ISSN 0936-6768 1439-0531 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2016 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Contents The objective of the present study was to investigate the effect of testicular tissue lysate ( TTL ) on developmental competence of germinal vesicle ( GV ) stage porcine oocytes. Two types of TTL were prepared through repeated freeze–thaw in liquid nitrogen, one from whole testicular tissue ( wTTL ) and other from either of four different sections of testes, namely just beneath the tunica albuginea ( TA ), from the transitional area between the seminiferous cord/tubules and the mediastinum testis ( TR ) and from the intermediate area (parenchymal tissue origin) and CE (cauda epididymis origin). The whole or section‐wise TTL treatments were given for 44 hr during in vitro maturation ( IVM ). Oocyte maturation was done in either of the two media, namely defined (high‐performance basic medium for porcine oocyte maturation, commercially available) and serum containing ( TCM 199). After maturation, oocytes were co‐incubated with fresh spermatozoa for 6 hr and then transferred to embryo culture media. Treatment of GV stage oocytes with wTTL (1 mg/ml) increased the cleavage and morula percentage rate (69.23 ± 6.23 and 48.15 ± 6.77, respectively) than that of their control (58.33 ± 8.08 and 32.54 ± 5.53, respectively) in defined media, and in serum‐containing media, cleavage and morula percentage rate were almost equal in both treatment (54.56 ± 7.79 and 34.70 ± 6.78, respectively) and control (59.52 ± 8.21 and 38.52 ± 6.54, respectively). However, effect of wTTL was not significant. In case of section‐wise TTL supplements, TR section significantly ( p < .01) improved cleavage and morula rate (58.43 ± 7.98 and 36.14 ± 6.89, respectively) followed by TA . In conclusion, present study indicates that IVM , in vitro fertilization and in vitro culture of embryo are improved in the presence of TTL , particularly its TR section. Further study is expected to reveal the principal components of TTL which may prove useful for IVM. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/rda.12875 |
| الاتاحة: | http://dx.doi.org/10.1111/rda.12875 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Frda.12875 https://onlinelibrary.wiley.com/doi/pdf/10.1111/rda.12875 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.CAA4948 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/rda.12875 |
|---|