Academic Journal
Determination and production of antimicrobial compounds by aspergillus clavatonanicus strain mj31, an endophytic fungus from mirabilis jalapa l. using uplc-esi-ms/ms and td-gc-ms analysis
| العنوان: | Determination and production of antimicrobial compounds by aspergillus clavatonanicus strain mj31, an endophytic fungus from mirabilis jalapa l. using uplc-esi-ms/ms and td-gc-ms analysis |
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| المؤلفون: | Mishra, Vineet Kumar, Passari, Ajit Kumar, Chandra, Preeti, Leo, Vincent Vineeth, Kumar, Brijesh, Uthandi, Sivakumar, Thankappan, Sugitha, Gupta, Vijai Kumar, Singh, Bhim Pratap |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2017 |
| المجموعة: | National University of Ireland (NUI), Galway: ARAN |
| مصطلحات موضوعية: | volatile organic-compounds, polyketide synthase genes, absolute-configuration, bioactive metabolites, microbial endophytes, antifungal activity, medicinal-plants, chemical ecology, trichoderma, diversity |
| الوصف: | Endophytic fungi associated with medicinal plants are reported as potent producers of diverse classes of secondary metabolites. In the present study, an endophytic fungi, Aspergillus clavatonanicus strain MJ31, exhibiting significant antimicrobial activity was isolated from roots of Mirabilis jalapa L., was identified by sequencing three nuclear genes i.e. internal transcribed spacers ribosomal RNA (ITS rRNA), 28S ribosomal RNA (28S rRNA) and translation elongation factor 1-alpha (EF 1 alpha). Ethyl acetate extract of strain MJ31 displayed significant antimicrobial potential against Bacillus subtilis, followed by Micrococccus luteus and Staphylococcus aureus with minimum inhibitory concentrations (MIC) of 0.078, 0.156 and 0.312 mg/ml respectively. In addition, the strain was evaluated for its ability to synthesize bioactive compounds by the amplification of polyketide synthase (PKS) and non ribosomal peptide synthetase (NRPS) genes. Further, seven antibiotics (miconazole, ketoconazole, fluconazole, ampicillin, streptomycin, chloramphenicol, and rifampicin) were detected and quantified using UPLC-ESI-MS/MS. Additionally, thermal desorption-gas chromatography mass spectrometry (TD-GC-MS) analysis of strain MJ31 showed the presence of 28 volatile compounds. This is the first report on A. clavatonanicus as an endophyte obtained from M. jalapa. We conclude that A. clavatonanicus strain MJ31 has prolific antimicrobial potential against both plant and human pathogens and can be exploited for the discovery of new antimicrobial compounds and could be an alternate source for the production of secondary metabolites. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1932-6203 |
| Relation: | PLOS ONE; Mishra, Vineet Kumar; Passari, Ajit Kumar; Chandra, Preeti; Leo, Vincent Vineeth; Kumar, Brijesh; Uthandi, Sivakumar; Thankappan, Sugitha; Gupta, Vijai Kumar; Singh, Bhim Pratap (2017). Determination and production of antimicrobial compounds by aspergillus clavatonanicus strain mj31, an endophytic fungus from mirabilis jalapa l. using uplc-esi-ms/ms and td-gc-ms analysis. PLOS ONE 12 (10); http://hdl.handle.net/10379/12918 |
| DOI: | 10.1371/journal.pone.0186234 |
| الاتاحة: | http://hdl.handle.net/10379/12918 https://doi.org/10.1371/journal.pone.0186234 |
| Rights: | Attribution-NonCommercial-NoDerivs 3.0 Ireland ; https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
| رقم الانضمام: | edsbas.CA699AC7 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0186234 |