Academic Journal
Impact of Divalent Cations on In-Layer Positional Order of DNA-Based Liquid Crystals: Implications for DNA Condensation
| العنوان: | Impact of Divalent Cations on In-Layer Positional Order of DNA-Based Liquid Crystals: Implications for DNA Condensation |
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| المؤلفون: | Sineth G. Kodikara, Prabesh Gyawali, James T. Gleeson, Antal Jákli, Samuel Sprunt, Hamza Balci |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Medicine, Molecular Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, using synchrotron small, ray scattering measurements, certain ionic conditions, based liquid crystals, 65 ° c, layer positional correlations, significantly enhanced even, physiologically relevant dna, layer positional order, layer dna ordering, 2 +, gapped dna constructs, 30 mm mg, 100 mm mg, fixed dna concentration, side interactions within, end stacking interactions, layer order, dna constructs, mm mg, even higher |
| الوصف: | The layered liquid crystalline phases formed by DNA molecules, which include rigid and flexible segments (“gapped DNA”), enable the study of both end-to-end stacking and side-to-side (helix-to-helix) lateral interactions, forming a model system to study such interactions at physiologically relevant DNA and ion concentrations. The observed layer structure exhibits long-range interlayer and in-layer positional correlations. In particular, the in-layer order has implications for DNA condensation, as it reflects whether these normally repulsive interactions become attractive under certain ionic conditions. Using synchrotron small-angle X-ray scattering measurements, we investigate the impact of divalent Mg 2+ cations (in addition to a constant 150 mM Na + ) on the stability of the inter- and in-layer DNA ordering as a function of temperature between 5 and 65 °C. DNA constructs with different terminal base pairings were created to mediate the strength of the attractive end-to-end stacking interactions between the blunt ends of the gapped DNA constructs. We demonstrate that the stabilities at a fixed DNA concentration of both interlayer and in-layer order are significantly enhanced even at a few mM Mg 2+ concentration. The stabilities are even higher at 30 mM Mg 2+ ; however, a marked decrease is observed at 100 mM Mg 2+ , suggesting a change in the nature of side-by-side interactions within this Mg 2+ concentration range. We discuss the implications of these results in terms of counterion-mediated DNA–DNA attraction and DNA condensation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Impact_of_Divalent_Cations_on_In-Layer_Positional_Order_of_DNA-Based_Liquid_Crystals_Implications_for_DNA_Condensation/24932958 |
| DOI: | 10.1021/acs.biomac.3c01086.s001 |
| الاتاحة: | https://doi.org/10.1021/acs.biomac.3c01086.s001 https://figshare.com/articles/journal_contribution/Impact_of_Divalent_Cations_on_In-Layer_Positional_Order_of_DNA-Based_Liquid_Crystals_Implications_for_DNA_Condensation/24932958 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.CA51219F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.biomac.3c01086.s001 |
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