Academic Journal
Spatially resolved protein map of intact human cytomegalovirus virions
| العنوان: | Spatially resolved protein map of intact human cytomegalovirus virions |
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| المؤلفون: | Bogdanow, Boris, Gruska, Iris, Mühlberg, Lars, Protze, Jonas, Hohensee, Svea, Vetter, Barbara, Bosse, Jens B., Lehmann, Martin, Sadeghi, Mohsen, Wiebusch, Lüder, Liu, Fan |
| سنة النشر: | 2023 |
| المجموعة: | Repository: Freie Universität Berlin (FU), Math Department (fu_mi_publications) |
| مصطلحات موضوعية: | Mathematical and Computer Sciences, Mathematics, Applied Mathematics |
| الوصف: | Herpesviruses assemble large enveloped particles that are difficult to characterize structurally due to their size, fragility and complex multilayered proteome with partially amorphous nature. Here we used crosslinking mass spectrometry and quantitative proteomics to derive a spatially resolved interactome map of intact human cytomegalovirus virions. This enabled the de novo allocation of 32 viral proteins into four spatially resolved virion layers, each organized by a dominant viral scaffold protein. The viral protein UL32 engages with all layers in an N-to-C-terminal radial orientation, bridging nucleocapsid to viral envelope. We observed the layer-specific incorporation of 82 host proteins, of which 39 are selectively recruited. We uncovered how UL32, by recruitment of PP-1 phosphatase, antagonizes binding to 14-3-3 proteins. This mechanism assures effective viral biogenesis, suggesting a perturbing role of UL32-14-3-3 interaction. Finally, we integrated these data into a coarse-grained model to provide global insights into the native configuration of virus and host protein interactions inside herpesvirions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | Bogdanow, Boris and Gruska, Iris and Mühlberg, Lars and Protze, Jonas and Hohensee, Svea and Vetter, Barbara and Bosse, Jens B. and Lehmann, Martin and Sadeghi, Mohsen and Wiebusch, Lüder and Liu, Fan (2023) Spatially resolved protein map of intact human cytomegalovirus virions. Nature Microbiology, 8 . pp. 1732-1747. |
| DOI: | 10.1038/s41564-023-01433-8 |
| الاتاحة: | http://publications.imp.fu-berlin.de/3039/ https://doi.org/10.1038/s41564-023-01433-8 |
| رقم الانضمام: | edsbas.C9C28600 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41564-023-01433-8 |
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