Academic Journal
16S rDNA Sequencing for Bacterial Identification in Preterm Infants with Suspected Early-Onset Neonatal Sepsis
| العنوان: | 16S rDNA Sequencing for Bacterial Identification in Preterm Infants with Suspected Early-Onset Neonatal Sepsis |
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| المؤلفون: | Agudelo-Pérez S, Moreno A.M, Martínez-Garro J, Salazar J, Lopez R, Perdigón M, Peláez R. |
| المصدر: | Universidad de La Sabana ; Intellectum Repositorio Universidad de La Sabana |
| بيانات النشر: | Tropical Medicine and Infectious Disease |
| سنة النشر: | 2024 |
| المجموعة: | Repositorio Universidad de La Sabana |
| مصطلحات موضوعية: | 16S ribosomal DNA gene, Antimicrobial stewardship, Early-onset neonatal sepsis, Preterm infant, Sanger sequencing |
| الوصف: | Background: The high prevalence of suspected early-onset neonatal sepsis among preterm infants leads to immediate antibiotic administration upon admission. Notably, most blood cultures for suspected early-onset neonatal sepsis do not yield a causative pathogen. This study aimed to assess polymerase chain reaction (PCR) targeting the variable region V4 of the 16S ribosomal gene (16S rDNA) and Sanger sequencing for bacterial identification in preterm infants with suspected early-onset neonatal sepsis. Methods: Therefore, this prospective study was conducted. Preterm infants with suspected early-onset neonatal sepsis were included in this study. The three groups were formed based on the risk of infection and clinical sepsis. Blood samples were collected upon admission to the neonatal unit for culture and molecular analysis. PCR amplification and subsequent Sanger sequencing of the V4 region of the 16S rDNA were performed. Results: Twenty-eight patients were included in this study. Blood cultures were negative in 100% of the patients. Amplification and sequencing of the V4 region identified bacterial genera in 19 patients across distinct groups. The predominant taxonomically identified genus was Pseudomonas. Conclusions: Amplifying the 16S rDNA variable region through PCR and subsequent Sanger sequencing in preterm neonates with suspected early-onset neonatal sepsis can enhance the identification of microbial species that cause infection, especially in negative cultures. © 2024 by the authors. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 24146366 |
| Relation: | Tropical Medicine and Infectious Disease Vol. 9 N° 7 art. 152; http://hdl.handle.net/10818/61938 |
| DOI: | 10.3390/tropicalmed9070152 |
| الاتاحة: | http://hdl.handle.net/10818/61938 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85199871464&doi=10.3390%2ftropicalmed9070152&partnerID=40&md5=ec4862a3551499ebb1caf0f36d205dae https://doi.org/10.3390/tropicalmed9070152 |
| Rights: | Attribution-NonCommercial-NoDerivatives 4.0 International ; http://creativecommons.org/licenses/by-nc-nd/4.0/ ; openAccess |
| رقم الانضمام: | edsbas.C9C1BC14 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 24146366 |
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| DOI: | 10.3390/tropicalmed9070152 |